20.500.12556/RUL-162092 Optimiziranje raztopine barvila Sudan IV za obarvanje premaznih sredstev pri pripravi mikroskopskih preparatov Optimization of the Sudan IV dye solution for staining of coating materials in the preparation of microscopic sections V diplomskem delu smo pripravljali različne raztopine barvila Sudan IV za obarvanje utrjenih filmov različnih premaznih sredstev pri pripravi mikroskopskih preparatov. Najprej smo se osredotočili na iskanje ustreznih topil. V naslednjem koraku smo pripravljene raztopine uporabili za obarvanje prostih filmov, pri čemer smo pri različnih časih pomakanja opazovali intenzivnost obarvanja in možno razkrajanje filmov. Najbolj optimalno raztopino smo nato uporabili še za končno obarvanje mikroskopskih preparatov. Za topila smo uporabili 70 % in 48 % etanol, 2-propanol, terpentin in vodo. V vodi se barvilo ni topilo. Najbolj intenzivno obarvanje smo dosegli z raztopino 2-propanol, vendar sta se vodna premaza v njem raztopila. Enako se je zgodilo tudi pri obarvanju z raztopino s terpentinom. Pri pomakanju v raztopini z 48 % etanolom smo dobili rahlo obarvanje filmov. Pri uporabi raztopine s 70 % etanolom pa smo že po 10 minutah dobili zelo intenzivno obarvanje filmov vseh premazov, do njihovega razkroja pa ni prišlo. Zaradi tega smo se odločili, da za obarvanje filmov premaznih sredstev pri pripravi mikroskopskih preparatov premazanega smrekovega lesa uporabimo samo to raztopino. Tako smo tudi praktično za obarvanje utrjenih filmov premazov dokazali vsestransko uporabnost oz. primernost rabe nasičene raztopine Sudana IV v 70 % etanolu s časom barvanja 10 minut. In this work, we prepared different solutions of Sudan IV dye for the staining of dry films of different coatings for the preparation of microscopic sections. First, we focussed on finding suitable solvents. In the next step, the prepared solutions were used to stain the dry free films, considering the intensity of the staining and the possible decay of the films at different immersion times. The optimal solution was then used for the final staining of the microscopic sections. We used 70% and 48% ethanol, 2-propanol, turpentine and water as the solvents. The dye did not dissolve in water. The most intensive colouring was achieved with a 2-propanol solution, but the water-borne coatings dissolved in it, as was also the case when staining with turpentine solution. When immersed in a solution containing 48% ethanol, a slight colouring of the films was achieved. When using a solution with 70% ethanol, a very intense colouring of the films of all coatings occurred after only 10 minutes, and films did not decompose. For this reason, we decided to use only this solution for staining coating films when preparing microscopic sections of coated spruce wood. In this way, we have practically demonstrated the comprehensive applicability and suitability for the staining of dry coating films using a saturated solution of Sudan IV in 70% ethanol with a treatment time of 10 minutes. les premaz sudan IV obarvanje wood coating sudan IV staining true false false [T. Čuk] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2024-09-19 07:16:31 2024-09-19 07:16:36 2024-10-03 08:56:11 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*829.18 245799 209995779 42421.pdf 42421.pdf 1 CF5C2365A6224100BFF8C2CC183D943B 891e50dac1bb2a2d28c37854de4ffe0f3c3c9e5e7a767db66bdd2b7edb29db4f 3c0b83e1-7646-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=191246 Biotehniška fakulteta 0 0 0