20.500.12556/RUL-161960 Long-term changes in transmembrane voltage after electroporation are governed by the interplay between nonselective leak current and ion channel activation Electroporation causes a temporal increase in cell membrane permeability and leads to prolonged changes in transmembrane voltage (TMV) in both excitable and non-excitable cells. However, the mechanisms of these TMV changes remain to be fully elucidated. To this end, we monitored TMV over 30 min after exposing two different cell lines to a single 100 µs electroporation pulse using the FLIPR Membrane Potential dye. In CHO-K1 cells, which express very low levels of endogenous ion channels, membrane depolarization following pulse exposure could be explained by nonselective leak current, which persists until the membrane reseals, enabling the cells to recover their resting TMV. In U-87 MG cells, which express many different ion channels, we unexpectedly observed membrane hyperpolarization following the initial depolarization phase, but only at 33 °C and not at 25 °C. We developed a theoretical model, supported by experiments with ion channel inhibitors, which indicated that hyperpolarization could largely be attributed to the activation of calcium-activated potassium channels. Ion channel activation, coupled with changes in TMV and intracellular calcium, participates in various physiological processes, including cell proliferation, differentiation, migration, and apoptosis. Therefore, our study suggests that ion channels could present a potential target for influencing the biological response after electroporation. electroporation transmembrane voltage calcium temperature ion channels theoretical model elektroporacija transmembranska napetost kalcij temperatura ionski kanali teoretični model true false true Angleški jezik Slovenski jezik Članek v reviji 2024-09-17 14:59:06 2024-09-17 14:59:13 2024-09-20 03:27:39 0000-00-00 00:00:00 2025 0 0 13 str. art. 108802 Vol. 161 Feb. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621:621.31 1567-5394 10.1016/j.bioelechem.2024.108802 207602179 1-s2.0-S1567539424001646-main.pdf 1-s2.0-S1567539424001646-main.pdf 1 709D53AE3CFC1A486CEBC78F7B0E88E6 82db0983da0a63da425a0a8b06d175bcc4a4b41c49bf38569370883e2a35b6ac d7965515-74f4-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190965 https://www.sciencedirect.com/science/article/pii/S1567539424001646 1 898f1cd7-74f4-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190964 Fakulteta za elektrotehniko 0 0 0