20.500.12556/RUL-161942 Raman microspectroscopy and laser-induced breakdown spectroscopy for the analysis of polyethylene microplastics in human soft tissues People are exposed to microplastics (MPs) on a large scale in everyday life. However, it is not clear whether MPs can also be distributed and retained in certain tissues. Therefore, the development of analytical methods capable of detecting MPs in specific human organs/tissues is of utmost importance. In this study, the use and combination of spectroscopic techniques, namely Raman microspectroscopy and laser-induced breakdown spectroscopy (LIBS), was tested for the detection of polyethylene (PE) MPs in human tonsils. Preliminary results showed that Raman microspectroscopy was able to detect MPs down to 1 μm in size and LIBS down to 10 μm. In the next step, human tonsils were spiked with PE MPs, and digested. The filtered particles were analyzed using Raman microspectroscopy and LIBS, and complemented by X-ray fluorescence (XRF). The results showed that Raman microspectroscopy could reliably detect PE MPs in spiked human tonsils, while LIBS and XRF served as a reference analytical method to characterize particles that could not be classified by Raman microspectroscopy for their non-organic origin. The results of this study, supported by a current feasibility study conducted on clinical samples, demonstrated the reliability and feasibility of this approach for monitoring MPs in biotic samples. plastics microplastics polyethylene tissue analysis human tonsils detection plastika mikroplastika polietilen analiza tkiva človeški mandeljni detekcija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-09-17 09:43:00 2024-09-17 09:43:03 2024-09-20 03:26:06 0000-00-00 00:00:00 2024 0 0 11 str. iss. 18, art. e37844 Vol. 10 30 Sep. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 504.5:678.742.2:543.42 2405-8440 10.1016/j.heliyon.2024.e37844 207627523 RAZ_Parobkova_Viktoria_2024.pdf RAZ_Parobkova_Viktoria_2024.pdf 1 9431F6F5BE9609163036E10C86B39D94 201239ae952b015efc539815ee79836d5e2270750111ecde0a44afd2ea668487 5bb021a0-74ca-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190942 https://www.sciencedirect.com/science/article/pii/S2405844024138753 1 5e3a69bf-74c8-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190940 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0