20.500.12556/RUL-161623 Sistem za merjenje temperaturnih gradientov v elektroporacijski kiveti System for measurement of temperature gradients in electroporation cuvettes Diplomsko delo obravnava razvoj in preskušanje sistema za natančno merjenje temperaturnih gradientov med postopkom elektroporacije v elektroporacijski kiveti. Elektroporacija je proces, ki uporablja močna električna polja za ustvarjanje prehodnih por v celičnih membranah za vnos molekul v celice. Za izboljšanje natančnosti in ponovljivosti meritev temperature znotraj kivete, je bil uporabljen optični temperaturni senzor v povezavi s po meri zasnovanim mikromanipulatorjem, natisnjenim s 3D tiskalnikom. Mikromanipulator zagotavlja tridimenzionalno pozicioniranje senzorja, kar omogoča odčitavanje temperature na poljubnih točkah znotraj kivete. Eksperimentalne meritve so pokazale znatne temperaturne razlike na različnih mestih v kiveti, kar poudarja pomen natančnega spremljanja in nadzora temperature. Čeprav so bile pri sestavnih delih mikromanipulatorja, opažene nekatere mehanske in strukturne omejitve, se je celoten sistem izkazal kot učinkovito orodje za merjenje temperature, kar potrjujejo dobljeni rezultati. This thesis addresses the development and testing of a novel system for precise measurement of temperature gradients during electroporation processes within an electroporation cuvette. Electroporation, a technique utilizing strong electric fields to create transient pores in cell membranes for molecular introduction, requires accurate temperature control for optimal results. To enhance the accuracy and reproducibility of temperature measurements, an optical temperature sensor was employed in conjunction with a custom-designed, 3D-printed micromanipulator. This setup allows for three-dimensional positioning of the sensor, enabling temperature readings at arbitrary points within the cuvette. Experimental measurements revealed significant temperature variations at different locations within the cuvette, underscoring the importance of precise temperature monitoring and control during electroporation procedures. While some mechanical and structural limitations were observed in the 3D-printed components, the overall system demonstrated efficacy as a temperature measurement tool, as evidenced by the obtained results. elektroporacija mikromanipulator temperaturni gradienti 3D tiskanje optični temperaturni senzor electroporation micromanipulator temperature gradients 3D printing optical temperature sensor true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2024-09-12 14:45:05 2024-09-12 14:45:09 2024-09-14 03:43:36 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 62539 207422467 Anzur_Gal_-_Sistem_za_merjenje_temperaturnih_gradientov_v_elektroporacijski_kiveti.pdf Anzur_Gal_-_Sistem_za_merjenje_temperaturnih_gradientov_v_elektroporacijski_kiveti.pdf 1 2EF5EA1A6A6DFA71B8B85D045CC64564 f2f289163ce28ce414a12cc0d1ce9aa38dcba09876b7168341d26cd4a800d3c2 cab870e8-7104-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190589 Fakulteta za elektrotehniko 0 0 0