20.500.12556/RUL-161265 Thermodynamic origin of the linear pressure dependence of DNA thermal stability High pressure affects the structure and function of DNA and is a key parameter for studying the origin and physical limits of life. Different types of DNA structures systematically show a linear pressure dependence of thermal stability (up to ∼200 MPa), which is maintained even when the solution composition is changed. The reasons behind the linear pressure dependence are not understood. We have performed a thermodynamic analysis of the pressure-, temperature- and composition-dependent (un)folding of various polynucleotide duplexes and G-quadruplexes. We demonstrate that the reason for the observed linearity is the link between compressibility and expansibility, both of which largely depend on DNA hydration. We predicted the temperature and pressure dependence of compressibility and expansibility of (un)folding and explain how they affect the corresponding volume change and thermodynamic stability parameters. These predictions indicate the existence of a convergence temperature at which compressibility and volume of (un)folding simultaneously become equal to zero. biomolecules DNA G-quadruplex stability pressure volume compressibility expansibility biopolymers genetics hydration biomolekule DNA G-kvadrupleks stabilnost tlak volumen stisljivost razteznost true false true Angleški jezik Slovenski jezik Članek v reviji 2024-09-09 08:53:54 2024-09-09 08:53:55 2025-08-08 13:41:37 0000-00-00 00:00:00 2024 0 0 Str. 9064-9069 iss. 35 Vol. 15 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.323 1948-7185 10.1021/acs.jpclett.4c01563 205693699 lah-hadzi-2024-thermodynamic-origin-of-the-linear-pressure-dependence-of-dna-thermal-stability.pdf lah-hadzi-2024-thermodynamic-origin-of-the-linear-pressure-dependence-of-dna-thermal-stability.pdf 1 29BC61FEE2B5FF2CDE55DC2A736C2ABA b6c4d8c8104e7211063fc588a60d6eb6cac59724efef7f0f49170b7cd3d5c750 7db7e08a-6e78-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190154 https://pubs.acs.org/doi/10.1021/acs.jpclett.4c01563 1 3e88df09-6e78-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190153 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0