20.500.12556/RUL-161258 Sinteza in ločba izomerov karboksirodamina B Synthesis and separation of isomers of carboxyrhodamine B V okviru diplomskega dela smo sintetizirali zmes izomerov 4'-karboksi- in 5'-karboksirodamina B 3a/3b ter raziskali metode za njuno ločbo. Za sintezo smo uporabili anhidrid 1,2,4-benzentrikarboksilne kisline in 3-(dietilamino)fenol. Po ekstrakciji reakcijske zmesi smo dobljeno zmes izomerov poskušali ločiti z različnimi metodami: kolonsko kromatografijo, preparativno tekočinsko kromatografijo srednje ločljivosti (MPLC) in preparativno tankoplastno kromatografijo. Kljub večkratnim poskusom ločb z različnimi kromatografskimi metodami, ločevanje ni bilo uspešno. Uporaba preparativne tankoplastne kromatografije pa se je izkazala za učinkovito pri ločbi izomerov. Izomera 3a in 3b smo identificirali s pomočjo NMR, infrardeče spektroskopije, ultravijolične in vidne spektroskopije, emisijske spektroskopije ter masne spektrometrije in ju primerjali z literaturnimi podatki. Ločbo smo uspešno izvedli in okarakterizirali izomera karboksirodamina B. In my work for a bachelor’s degree, the isomers of 4'-carboxy- and 5'-carboxyrhodamine B 3a/3b were synthesized and methods for their separation were investigated. The synthesis was performed from 1,2,4-benzenetricarboxylic anhydride and 3-(diethylamino)phenol. Following the extraction of the product from the reaction mixture, various methods were employed in an attempt to separate the resulting mixture of isomers: column chromatography, medium pressure liquid chromatography (MPLC) and preparative thin-layer chromatography. Despite multiple attempts with different chromatographic methods, the separation was initially unsuccessful. However, the use of preparative thin-layer chromatography proved effective in separating the isomers. The identification of isomers 3a and 3b was carried out using NMR, infrared spectroscopy, ultraviolet-visible spectroscopy, emission spectroscopy and mass spectrometry. These results were then compared with existing literature data. The separation was successfully achieved and the isomers of carboxyrhodamine B were characterized. karboksirodamin B sinteza izomerov ločba izomerov kolonska kromatografija tankoplastna kromatografija carboxyrhodamine B synthesis of isomers separation of isomers column chromatography thin-layer chromatography true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2024-09-09 08:11:48 2024-09-09 08:11:51 2024-09-12 13:36:21 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 24283 207318019 Gasperlin_Spela_-_Sinteza_in_locba_izomerov_karboksirodamina_B.pdf Gasperlin_Spela_-_Sinteza_in_locba_izomerov_karboksirodamina_B.pdf 1 78EE2B6BF251D77DDBB069A9410266F8 2932b089cb02b7a9bdfcce3079c93bdfff83d58e9048c4554caabc555c218da7 5e4de2e0-6e72-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=190146 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0