20.500.12556/RUL-160683 Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditions Aerobic respiration is the main energy source for most eukaryotes, and efficient mitochondrial energy transfer greatly influences organismal fitness. To survive environmental changes, cells have evolved to adjust their biochemistry. Thus, measuring energy metabolism at the subcellular level can enhance our understanding of individual performance, population dynamics, and species distribution ranges. We investigated three important metabolic traits at the subcellular level in five lacertid lizard species sampled from different elevations, from sea level up to 2000 m. We examined hemoglobin concentration, two markers of oxidative stress (catalase activity and carbonyl concentration) and maximum rate of metabolic respiration at the subcellular level (potential metabolic activity at the electron transport system). The traits were analysed in laboratory acclimated adult male lizards to investigate the adaptive metabolic responses to the variable environmental conditions at the local sampling sites. Potential metabolic activity at the cellular level was measured at four temperatures – 28 °C, 30 °C, 32 °C and 34 °C – covering the range of preferred body temperatures of the species studied. Hemoglobin content, carbonyl concentration and potential metabolic activity did not differ significantly among species. Interspecific differences were found in the catalase activity, Potential metabolic activity increased with temperature in parallel in all five species. The highest response of the metabolic rate with temperature (Q$_{10}$) and Arrhenius activation energy (E$_a$) was recorded in the high-mountain species Iberolacerta monticola. physiology lacertids aerobic respiration interspecific variability hemoglobin zoology fiziologija kuščarice aerobna respiracija medvrstna variabilnost hemoglobin zoologija Lacertidae true false true Angleški jezik Slovenski jezik Članek v reviji 2024-09-03 11:24:17 2024-09-03 11:24:25 2024-09-04 04:00:35 0000-00-00 00:00:00 2024 0 0 10 str. art. 111729 Vol. 297 Nov. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 591 1095-6433 10.1016/j.cbpa.2024.111729 206128131 1-s2.0-S1095643324001569-main.pdf 1-s2.0-S1095643324001569-main.pdf 1 803343097CD6F84477FF871B7D084E83 915438d94f10db9cf05c04d334a7c091615a9c14cc18ca7891d3c7e9eff94617 6925de2e-69d6-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=189448 https://www.sciencedirect.com/science/article/pii/S1095643324001569 0 3fb31e89-69d6-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=189447 Biotehniška fakulteta 0 0 0