20.500.12556/RUL-160551 Vpliv epigenetskih dejavnikov na odpornost rastlin proti boleznim Influence of epigenetic factors on plant disease resistance Diplomska naloga opisuje molekularne in epigenetske mehanizme, ki prispevajo k prilagoditvi rastlin na stresne pogoje, s posebnim poudarkom na sušnih razmerah. Namen raziskave je bil ugotoviti, kako lahko epigenetske spremembe, kot je metilacija DNA, vplivajo na fenotipsko plastičnost rastlin ter ali te spremembe postanejo dedne in trajne skozi več generacij. Podatke so pridobili z eksperimenti izvedenimi s kontroliranim izpostavljanjem rastlin blagim stresnim pogojem, medtem ko so bile molekularne spremembe spremljane z naprednimi tehnikami, kot so analize izražanja genov in epigenetskih vzorcev. Ugotovljeno je bilo, da rastline razvijejo prilagoditve na sušne pogoje, vendar te epigenetske spremembe niso nujno trajne in se ne prenašajo vedno na naslednje generacije. Naloga nadalje analizira potencialne aplikacije teh ugotovitev v kmetijstvu, vključno z možnostjo selekcije rastlin, ki so bolj odporne na okoljske stresne dejavnike. Rezultati raziskave nakazujejo na omejeno transgeneracijsko dednost epigenetskih sprememb, kar ima pomembne posledice za razvoj novih strategij za izboljšanje pridelkov in trajnostne kmetijske prakse. Opisane raziskave odpirajo pot za nadaljnje študije, ki bi se osredotočile na dolgoročne učinke epigenetskih modifikacij in njihovo vlogo pri prilagajanju rastlin na spreminjajoče se okoljske pogoje. This thesis describes the molecular and epigenetic mechanisms that contribute to plant adaptation to stress conditions, with a particular focus on drought environments. The aim of the research was to determine how epigenetic changes, such as DNA methylation, affect the phenotypic plasticity of plants and whether these changes become heritable and persistent across multiple generations. Described experiments were conducted by subjecting plants to controlled mild stress conditions while monitoring molecular changes using advanced techniques such as gene expression analysis and epigenetic pattern profiling. It was found that plants develop adaptations to drought conditions, but these epigenetic changes are not necessarily permanent and do not always transmit to subsequent generations. The thesis further examines the potential applications of these findings in agriculture, including the possibility of selecting plants that are more resilient to environmental stressors. The described research results indicate a limited transgenerational inheritance of epigenetic changes, which has significant implications for developing new strategies to improve crop yields and promote sustainable agricultural practices. Described studies pave the way for further research focusing on the long-term effects of epigenetic modifications and their role in plant adaptation to changing environmental conditions. epigenetika rastline odpornost proti boleznim DNA metilacija histonske modifikacije RNA interferenca imunski sistem rastlin epigenetics plants disease resistance DNA methylation histone modifications RNA interference plant immune system true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2024-08-31 07:15:52 2024-08-31 07:16:00 2024-09-03 09:43:46 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 242113 206164739 41521.pdf 41521.pdf 1 FE8E2AC4DABCED957BE09484A9DE53C4 a09543426ecebe7598e99d369c0ea94c8821d0ba3323a496fce05f3e6a93d409 df9b1649-6757-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=189281 Biotehniška fakulteta 0 0 0