20.500.12556/RUL-160227 Changes in mitochondrial membrane potential in in vitro electroporation with nano- and microsecond pulses Tamara Polajžer ... [et al.] With the introduction of nanosecond (ns) pulses, it was suggested that such pulses could be used to permeabilize intracellular membranes, including the mitochondrial membrane. The results presented thus far, however, are not conclusive. Interestingly, the effect of longer microsecond (▫$\mu$▫s) pulses on changes in mitochondria has never been investigated. We, therefore, investigated the changes in mitochondrial membrane permeability through changes in mitochondrial membrane potential (MMP) in CHO and H9c2 cells after electroporation with 4 ns, 200 ns, and 100 ▫$\mu$▫s pulses. In the range of reversible electroporation, the decrease in MMP generally depended on the cell line. In CHO, ns pulses decreased MMP at lower electroporation intensities than μs. In H9c2, ns and ▫$\mu$▫s were equally effective. In the range of irreversible electroporation, MMP decreased even further, regardless of pulse duration and cell type. The analysis at different time points showed that the changes in MMP within the first hour after pulse treatment are dynamic. Our results on the efficacy of ns pulses are consistent with published data, but with this study we show that ▫$\mu$▫s pulses cause similar changes in MMP as ns pulses, demonstrating that electroporation affects MMP regardless of pulse duration. At the same time, however, differences in MMP changes were observed between different cell lines, indicating some dependence of MMP changes on cell type. electroporation nanosecond pulses microsecond pulses mitochondria mitochondrial membranepotential elektroporacija nanosekundni pulzi mikrosekundni pulzi mitohondriji mitohondrijski membranski potencial true false true Mary Ann Liebert Angleški jezik Slovenski jezik Članek v reviji 2024-08-23 12:53:21 2024-08-23 12:53:24 2025-06-30 10:56:26 0000-00-00 00:00:00 2024 0 0 Str. 97-107 iss. 2 Vol. 6 Jun. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 602.621 2576-3113 10.1089/bioe.2024.0007 200294659 polajzer-et-al-2024-changes-in-mitochondrial-membrane-potential-in-in-vitro-electroporation-with-nano-and-microsecond.pdf polajzer-et-al-2024-changes-in-mitochondrial-membrane-potential-in-in-vitro-electroporation-with-nano-and-microsecond.pdf 1 FEA29B23BAF6F922C6F74C462442A2DC 687683e95461f516e790623138a5d1d226fcd223aceb1d4baed1f9230de9e6e4 87f18c99-613e-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188796 https://www.liebertpub.com/doi/full/10.1089/bioe.2024.0007 1 b451c4c7-613d-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188794 Fakulteta za elektrotehniko 0 0 0