20.500.12556/RUL-160097 Evaluation of THP-1 and Jurkat cell lines coculture for the in vitro assessment of the effects of immunosuppressive substances The strong appeal to reduce animal testing calls for the development and validation of in vitro, in chemico and in silico models that would replace the need for in vivo testing and ex vivo materials. A category that requires such new approach methods is the assessment of immunosuppression that can be induced by chemicals including environmental pollutants. To assess the immunosuppressive action on monocytes and lymphocytes, we mimicked the whole-blood cytokine-release assay by preparing an in vitro coculture of THP-1 and Jurkat cell lines. We optimised its activation and investigated the effects of known immunosuppressive drugs with different mechanisms of action on the release of proinflammatory cytokines. Decreased secretion of IL-8 was achieved by several immunosuppressive mechanisms and was therefore selected as an appropriate marker of immunosuppression. A set of environmentally occurring bisphenols, BPA, BPAP, BPP, BPZ, BPE, TCBPA and BPS-MAE, were then applied to the model and BPP and BPZ were found to act as potent immunosuppressants at micromolar concentrations. immunosuppression coculture in vitro new approach method cell lines bisphenols imunosupresija kokultura in vitro metoda novega pristopa celične linije bisfenoli imunosupresivna biološka zdravila hormonski motilci true false true Angleški jezik Slovenski jezik Članek v reviji 2024-08-20 10:59:57 2024-08-20 11:00:00 2025-02-03 08:41:42 0000-00-00 00:00:00 2024 0 0 13 str. iss. 8, art. 607 Vol. 12 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.37:612.43:620.266.1 2305-6304 10.3390/toxics12080607 204624131 toxics-12-00607-v2.pdf toxics-12-00607-v2.pdf 1 EDE47FAF4EC0F92E303E6C95263D98CE 763702be88fcfe8b81514d31f3423466828a6a1e0231550e9a011801d90aba00 bfb6d17c-5ed2-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188561 https://www.mdpi.com/2305-6304/12/8/607 1 61c06f8d-5ed2-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188560 Fakulteta za farmacijo 0 0 0