20.500.12556/RUL-160055 Effect of castration method on porcine skeletal muscle fiber traits and transcriptome profiles This study examined the effects of immunocastration and surgical castration on the histomorphometric and transcriptome traits of the porcine skeletal muscle. We hypothesized that the differences in duration of androgen deprivation resulting from different castration methods influence skeletal muscle biology in a muscle-specific manner. This was tested by analyzing samples of m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) from immunocastrated (IC; n = 12), entire male (EM; n = 12), and surgically castrated (SC; n = 12) pigs using enzyme/immunohistochemical classification and histomorphometric analysis of myofibers, quantitative PCR, and RNA sequencing. The results confirmed the distinctive histomorphometric profiles of LD and SSC and the castration method related muscle-specific effects at the histomorphometric and transcriptome levels. Long-term androgen deficiency (surgical castration) significantly reduced the proportion of fast-twitch type IIa myofibers in LD (P < 0.05), whereas short-term androgen deprivation (immunocastration) reduced the cross-sectional area of oxidative type I myofibers in SSC (P < 0.05). At the transcriptional level, glycolytic LD adapted to long- and short-term androgen deprivation by upregulating genes controlling myoblast proliferation and differentiation to maintain fiber size. In contrast, increased protein degradation through the ubiquitin ligase-mediated atrophy pathway (significantly increased TRIM63 and FBXO32 expression; P < 0.05) could underly reduced crosssectional area of type I myofibers in the oxidative SSC in IC. Potential candidate genes (HK2, ARID5B, SERPINE1, and SCD) linked to specific metabolic profiles and meat quality traits were also identified in IC, providing a foundation for studying the effects of immunocastration on skeletal muscle fiber and carcass/meat quality traits. sceletal muscle Logissimus dorsi Semispinalis capitis castration method genne expression pig true false true Angleški jezik Ni določen Članek v reviji 2024-08-13 12:00:44 2024-08-13 12:00:50 2024-08-14 04:17:44 0000-00-00 00:00:00 2024 0 0 13 str. Vol. 25, art. 100383 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.4.09:611 2451-943X 10.1016/j.vas.2024.100383 203362563 Effect_of_castration-Fazarinc.pdf Effect_of_castration-Fazarinc.pdf 1 9FE27A8B790F38D696D081D232C54E76 8f865f3b16d6d8e78ad4d374fb496d4f39379ff7733539dc952198f47231313f 3c358ee3-595c-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188479 https://www.sciencedirect.com/science/article/pii/S2451943X24000504?via%3Dihub 1 c09aab55-595a-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188478 Veterinarska fakulteta 0 0 0