20.500.12556/RUL-159798 Simultaneous measurement of 17 endogenous steroid hormones in human serum by liquid chromatography-tandem mass spectrometry without derivatization Mass spectrometric-based steroidomics is a valuable analytical approach that gives a comprehensive understanding of the interlinked steroid biosynthetic pathways. Here, we describe a rapid and versatile liquid chromatography-tandem mass spectrometry (LC-MS/MS) method designed to accurately quantify endogenous steroids in human serum. Sample preparation involved liquid-liquid extraction with methyl tert-butyl ether (MTBE) from 180 µL serum. The targeted steroids for quantification included androgens: dehydroepiandrosterone (DHEA), androstenedione (A4), testosterone (T), dihydrotestosterone (DHT), 11-oxyandrogens: 11β-hydroxy-androstenedione (11OHA4), 11-keto-androstenedione (11KA4), 11β-hydroxy-testosterone (11OHT), 11-keto-testosterone (11KT), progestogens: 17α-hydroxy-progesterone (17OHP4), progesterone (P4), 11β-hydroxy-progesterone (11OHP4), 11-keto-progesterone (11KP4), mineralocorticoids: aldosterone, corticosterone, and glucocorticoids: 11-deoxycortisol, cortisol, and cortisone. The lower limits of quantification (LLOQ) were 0.05 ng/mL for A4, T, 11KA4, P4, and cortisone, 0.1 ng/mL for DHT, 11OHA4, 11OHT, 11KT, 17OHP4, 11OHP4, 11KP4, corticosterone, aldosterone, 11-deoxycortisol, and cortisol, and 0.5 ng/mL for DHEA. Accuracy, precision, reproducibility, and recovery fell within acceptable limits for bioanalytical method validation. Using serum samples from 29 premenopausal women in different menstrual phases, we demonstrated the clinical utility of our method, which showed sufficient sensitivity to reliably quantify all targeted steroids at levels typically found in circulation, except for 11OHP4 and 11KP4. liquid chromatography-tandem mass spectrometry androgens progestogens mineralocorticoids glucocorticoids 11-oxygenenated steroids tekočinska kromatografija s tandemsko masno spektrometrijo androgeni gestageni mineralokortikoidi glukokortikoidi 11-oksigenirani steroidi true false true Angleški jezik Slovenski jezik Članek v reviji 2024-07-25 10:06:25 2024-07-25 10:06:30 2024-07-27 03:26:51 0000-00-00 00:00:00 2024 0 0 8 str. Vol. 243, art. 106578 Oct. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 1879-1220 10.1016/j.jsbmb.2024.106578 202258435 1-s2.0-S0960076024001262-main.pdf 1-s2.0-S0960076024001262-main.pdf 1 16F8A6561F38FA1D78127AF6B619ECC2 892da056228f1758780cd62aa49c6377c6c1da82759f310c4609a439d402154e ed8092ca-4a5c-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188005 https://www.sciencedirect.com/science/article/pii/S0960076024001262 0 b5b10cbd-4a5c-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=188004 Medicinska fakulteta 0 0 0