20.500.12556/RUL-159659 Investigating the effect of ammonium fluoride as an eluent modifier on the response signal of vitamins research data Method Development The most common technique for developing methods to analyze vitamins is an HPLC system coupled with a mass spectrometer (HPLC-MS). A triple quadrupole mass spectrometer operating under multiple reaction mode was used for analysis. At least two mass transitions per vitamin were selected and optimized for the corresponding ESI-MS/MS parameters. The details of the HPLC-MS/MS method can be found in the published articles. Method Validation According to the definition presented in ISO 5725, accuracy has two main components, trueness and precision, which must be assessed during the validation of an analytical method (International Organization for Standardization, 2020). These two characteristics can be evaluated holistically using the concept of accuracy profiles. The first step in method validation with accuracy profiles is deciding the acceptability limit (λ) which was set at 25 % in the current study. The following step is determining measurement uncertainty in the bias value at different concentration levels that cover the expected concentration range of target analytes (vitamins) in target samples (fruit juices). The data files summarizing validation results for different vitamins in different matrices (standard solution, strawberry nectar, and multifruit juice) are in Excel file format. They have three sheets inside each data file. The first three sheets contain the raw data from the different days the calibration or validation study was carried out. Please note that the concentrations are reported in µg/L, unless otherwise stated. Please refer to the coding scheme for samples in Figure 1 and Figure 2 for more details about the sample identity. Effect of Ammonium Fluoride as an Eluent Modifier on the Response Signal of Vitamins There are two excel files corresponding to the objective of exploring the effect of ammonium fluoride on the response signal of vitamins. The Excel file “2024 07 08_Effect of Ammonium flouride on B vitamins_rawdata” summarizes data on all B vitamins' response signals in standard solutions. The response signal is measured without ammonium fluoride, 0.5 mM, and 1.5 mM ammonium fluoride. The Excel file “2024 07 10_B7B12_effect of ammonium fluoride on LOQ” summarizes the results for the effect of ammonium fluoride on the response signal of biotin (B7) and cyanocobalamin (B12) in selected fruit extracts. The coding scheme for the samples can be found in Figure 3. Comparison of Technologies in Terms of Vitamin Retention The developed methods for B vitamins and vitamin C were used to determine the vitamin content in differently treated strawberry nectar (conventional thermal processing-TT, ohmic processing-OH, pulse electric field-PEF, high pressure processing-HPP). The analysis was done in triplicate. ammonium fluoride limit of quantitation recovery biotin cyanocobalamin true false false Angleški jezik Ni določen Neznano 2024-07-17 16:13:51 2024-07-17 16:13:51 2025-04-16 13:35:14 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 Description_of_data_sets_05.06.2024.docx Description_of_data_sets_05.06.2024.docx Brief description of data 1 B4A608CE10387FB9B726FF14BA385E4D 4205b853c65d92551a33528f504c493f4b49b25cdf691aa0ea35833124352e3a 618d5bee-4447-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187782 Effect_of_Ammonium_fluoride_description_+_Data_extract.docx Effect_of_Ammonium_fluoride_description_+_Data_extract.docx Figure, graph 1 B87DB52B4746B12DA62C68F6A7E9BAA3 8d472ef0fd6e1e2589c648cd4bdbc075e2c8b47716ac9a2333031c25d83710ba 8db8a6d5-4447-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187783 2024_07_08_Effect_of_Ammonium_flouride_on_B_vitamins_rawdata.xlsx 2024_07_08_Effect_of_Ammonium_flouride_on_B_vitamins_rawdata.xlsx 1 2B9AF5DF00EA0767CCB24E4BC0BE37E0 7caa422c3a514bc83cad14cb3346a689ef43a4059e5a70f8a192b96cba7e6458 a54994ba-4447-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187784 2024_07_10_B7B12_effect_of_ammonium_fluoride_on_LOQ.xlsx 2024_07_10_B7B12_effect_of_ammonium_fluoride_on_LOQ.xlsx 1 B60CA3CEE5D6AA4031C1B972BB1B2986 bd7d292d113c9f717c2c9ad98034c21bbfefa2d0a9976c205d53c0fd44d14432 ae9b350b-4447-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187785 Biotehniška fakulteta Meritve in testi 0 0 0