20.500.12556/RUL-159566 Supplementation of vitamin E as an addition to a commercial renal diet does not prolong survival of cats with chronic kidney disease Background The aim of this double-blind, placebo-controlled study was to investigate the effect of vitamin E supplementation as an addition to a commercial renal diet on survival time of cats with different stages of chronic kidney disease (CKD). In addition, we were interested whether vitamin E supplementation affects selected oxidative stress and clinical parameters. Thirty-four cats with CKD and 38 healthy cats were included in the study. Cats with CKD were classified according to the IRIS Guidelines; seven in IRIS stage 1, 15 in IRIS stage 2, five in IRIS stage 3 and seven in IRIS stage 4. Cats with CKD were treated according to IRIS Guidelines. Cats with CKD were randomly assigned to receive vitamin E (100 IU/cat/day) or placebo (mineral oil) for 24 weeks in addition to standard therapy. Plasma malondialdehyde (MDA) and protein carbonyl (PC) concentrations, DNA damage of peripheral lymphocytes and plasma vitamin E concentrations were measured at baseline and four, eight, 16 and 24 weeks thereafter. Routine laboratory analyses and assessment of clinical signs were performed at each visit. Results Vitamin E supplementation had no effect on the survival time and did not reduce the severity of clinical signs. Before vitamin E supplementation, no significant differences in vitamin E, MDA and PC concentrations were found between healthy and CKD cats. However, plasma MDA concentration was statistically significantly higher (p = 0.043) in cats with early CKD (IRIS stages 1 and 2) than in cats with advanced CKD (IRIS stages 3 and 4). Additionally, DNA damage was statistically significantly higher in healthy cats (p ≤ 0.001) than in CKD cats. Plasma vitamin E concentrations increased statistically significantly in the vitamin E group compared to the placebo group four (p = 0.013) and eight (p = 0.017) weeks after the start of vitamin E supplementation. During the study and after 24 weeks of vitamin E supplementation, plasma MDA and PC concentrations and DNA damage remained similar to pre- supplementation levels in both the placebo and vitamin E groups. Conclusions Vitamin E supplementation as an addition to standard therapy does not prolong survival in feline CKD. chronic renal insufficiency oxidative stress vitamin E survival chronic kidney disease true false true Angleški jezik Ni določen Članek v reviji 2024-07-12 13:00:23 2024-07-12 13:00:27 2025-01-22 15:05:25 0000-00-00 00:00:00 2024 0 0 13 str. Vol. 20, art. 308 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616 1746-6148 10.1186/s12917-024-04176-8 201505027 Supplementation-Krofic.pdf Supplementation-Krofic.pdf 1 55F8BA98967C2D91174DE0C7A80363C2 0e4018176d76e5467609b4b64fabf2fac6dc4ce345dc1c4a9f92c08ddc4f0bed b6d92e4c-4040-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187619 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04176-8 1 ed724b3d-403d-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187617 Veterinarska fakulteta Fakulteta za farmacijo Medicinska fakulteta 0 0 0