20.500.12556/RUL-159513 Vpliv z antioksidanti bogate prehrane v vrtcih na oksidativni stres pri zdravih otrocih The effect of antioxidant-rich kindergarten diet on oxidative stress in healthy children V Sloveniji je v vrtce vključenih več kot 90 % 4–6-letnih otrok, kjer z obroki zaužijejo do 75 % dnevnih energijskih in hranilnih potreb. Glavni namen raziskovalnega dela je bil raziskati, ali lahko 10-dnevna, z antioksidanti bogata vrtčevska prehrana prispeva k znižanju označevalcev oksidativnega stresa pri zdravih otrocih. V raziskavo smo vključili 57 zdravih otrok v starosti 5–6 let, iz 6 vrtcev in jih randomizirali v prototipno (n=40; 4 vrtci) in kontrolno skupino (n=17; 2 vrtca). Na podlagi kemijske analize skupne antioksidativne kapacitete, vsebnosti energije, makrohranil in izbranih elementov v posameznih 5-dnevnih vrtčevskih obrokih, na podlagi s spletnim orodjem za ocenjevanje prehrane (OPKP) ovrednotene hranilne vrednosti 7-dnevnih tehtanih prehranskih dnevnikov iz vrtca in izven vrtca in na podlagi laboratorijskih analiz vzorcev urina pred in po intervencijski prehrani ter vzorcev krvi po intervencijski prehrani smo primerjali prispevek z antioksidanti bogatih vrtčevskih obrokov (prototipni obroki) s prispevkom kontrolnih vrtčevskih obrokov, na označevalce oksidativnega stresa pri udeležencih. Rezultati raziskave so pokazali, da je dobro načrtovana in natančno izvedena vrtčevska prehrana bogata z antioksidanti, v primerjavi s kontrolno vrtčevsko prehrano, prispevala k bolj optimalnim dnevnim vnosom zdravih skupin živil pri udeležencih, k bolj optimalni prehranski kakovosti pripravljenih obrokov in prispevala k visokemu skupnemu vnosu prehranskih antioksidantov pri udeležencih raziskave. Vse to se je odrazilo v signifikantnem znižanju 8-izo-15-prostaglandina-F-2 alfa v urinu udeležencev prototipne skupine po končani intervenciji in v signifikantno negativni korelaciji med vnosom prehranskih antioksidantov in serumskimi označevalci oksidativnega stresa. Naše ugotovitve močno spodbujajo ponujanje z antioksidanti bogate prehrane v vrtcu, zaradi njenega potencialnega prispevka k varovanju zdravja. In Slovenia, over 90 % of 4–6-year-old children are enrolled in kindergartens, where they consume up to 75 % of their daily energy and nutritional needs. The main purpose of the current research was to evaluate whether 10-day, antioxidant-rich kindergarten meals can contribute to the reduction of oxidative stress biomarkers in healthy children. The study included 57 healthy children aged 5–6 years from 6 kindergartens, randomized into a prototype group (n=40, n=4) and a control group (n=17, n=2). Based on the chemical analysis of the total antioxidant capacity, energy content, macronutrients, and selected elements in individual 5-day kindergarten meals, analyses of consecutive 7-day dietary records inside and outside kindergarten, using a dietary assessment tool (OPEN), and based on laboratory analyses of urine samples before and after the intervention diet, as well as blood samples after the intervention diet, we compared the contribution of the antioxidant-rich kindergarten meals (prototype meals) with the contribution of control kindergarten meals on oxidative stress biomarkers in participants. The results showed that a well-designed and precisely executed antioxidant-rich kindergarten meals contributed to a more optimal intakes of healthy food groups in the participants, enhanced the nutritional quality of prepared meals, and contributed to a higher total intake of dietary antioxidants in healthy participants. All this was reflected in a significant decrease in 8-iso-15-prostaglandin-F-2 alpha in the urine of prototype group participants after intervention and in a significant negative correlation between the intake of dietary antioxidants and serum markers of oxidative stress. Our findings support the provision of antioxidant-rich foods in kindergartens due to their potential health-protective contribution. prehrana prehranske smernice predšolski otroci vrtčevski obroki dnevni vnos živil načrtovanje obrokov organiziran sistem šolske prehrane kemijska analiza hranilna vrednost antioksidanti skupna antioksidativna kapaciteta oksidativni stres nutrition dietary guidelines preschool children kindergarten meals daily food intake meal planning organized school diet system chemical analysis nutritional value antioxidants total antioxidant capacity oxidative stress true false false [M. Berlic] Slovenski jezik Angleški jezik Doktorsko delo/naloga 2024-07-11 10:44:15 2024-07-11 10:44:23 2024-07-17 03:44:47 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 613.2-053.4:641.1:66.094.3.097.8 236955 201638403 40073.pdf 40073.pdf 1 B3B6FE1110DBC8E227353A3CD9661E7E aae9463fa4f6ab722e16ac2d9df93817a7aa239226c3b41b4cf3272d6119e835 c21d0743-3f61-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187545 Biotehniška fakulteta 0 0 0