20.500.12556/RUL-159298 Immobilization of ▫$His_6$▫-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes Enzyme immobilization is one of the essential tools that enables bioprocess intensification as it facilitates reuse of enzyme. Nanomaterials can be used as supports for enzyme immobilization because of their high surface to volume ratio. In his work a designed biocompatible non-woven nanofiber was used as a support for hexahistidine tagged amine transaminase immobilization in a microreactor. Four different nanofibers were tested and the one with surface immobilized Cu$^{2+}$ ions was proven to be the best. Additionally, direct immobilization of overexpressed enzymes in E. coli cell lysate was performed and yielded loads up to 1088 U mL$^{-1}$. The highest turnover number reached within the indicated time was 7.23 10$^6$. enzyme immobilization amine transaminase microreactor membrane imobilizacija encimov amin transaminaze mikroreaktor membrana true false true Angleški jezik Slovenski jezik Članek v reviji 2024-07-05 10:09:34 2024-07-05 10:09:34 2024-07-15 03:18:09 0000-00-00 00:00:00 2024 0 0 Str. 46-55 Vol. 83 25 Nov. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.098 1871-6784 10.1016/j.nbt.2024.06.005 200710147 RAZ_Sketa_Borut_2024.pdf RAZ_Sketa_Borut_2024.pdf 1 F8D464BD043BA03263FC09CDBD3299FA e26e7eca854e0df661d528bb405a584b5eca241c860d4577518b023c9fe7f701 da617b5a-3aa6-11ef-89ef-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187265 https://www.sciencedirect.com/science/article/pii/S1871678424000268 1 ea03a57f-3aa5-11ef-89ef-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=187264 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0