20.500.12556/RUL-158969 DNA preservation in compact and trabecular bone Significant variation exists in the molecular structure of compact and trabecular bone. In compact bone full dissolution of the bone powder is required to efficiently release the DNA from hydroxyapatite. In trabecular bone where soft tissues are preserved, we assume that full dissolution of the bone powder is not required to release the DNA from collagen. To investigate this issue, research was performed on 45 Second World War diaphysis (compact bone)-epiphysis (trabecular bone) femur pairs, each processed with a full dissolution (FD) and partial dissolution (PD) extraction method. DNA quality and quantity were assessed using qPCR PowerQuant analyses, and autosomal STRs were typed to confirm the authenticity of isolated DNA. Our results support different mechanisms of DNA preservation in compact and trabecular bone because FD method was more efficient than PD method only in compact bone, and no difference in DNA yield was observed in trabecular bone, showing no need for full dissolution of the bone powder when trabecular bone tissue is processed. In addition, a significant difference in DNA yield was observed between compact and trabecular bone when PD was applied, with more DNA extracted from trabecular bone than compact bone. High suitability of trabecular bone processed with PD method is also supported by the similar quantities of DNA isolated by FD method when applied to both compact and trabecular bone. Additionally similar quantities of DNA were isolated when compact bone was extracted with FD method and trabecular bone was extracted with PD method. Processing trabecular bone with PD method in routine identification of skeletonized human remains shortens the extraction procedure and simplifies the grinding process. aged DNA compact bone DNA preservation human identification trabecular bone stara DNA kompaktna kost ohranjanje DNA identifikacija človeka trabekularna kost true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-24 11:47:04 2024-06-24 11:47:09 2026-06-18 13:17:13 0000-00-00 00:00:00 2024 0 0 6 str. Vol. 71, art. 103067 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61:575 1878-0326 10.1016/j.fsigen.2024.103067 199642627 1-s2.0-S1872497324000632-main.pdf 1-s2.0-S1872497324000632-main.pdf 1 8237B78E7B2F915CA5670020136F831F 2693538da92c10dc58771011503e530c12138a1b94f58bf2193576b6acc9c865 4a848b52-320f-11ef-b16a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186706 https://www.sciencedirect.com/science/article/pii/S1872497324000632 0 1c54c9b4-320f-11ef-b16a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186705 Medicinska fakulteta 0 0 0