20.500.12556/RUL-158802 Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM. type 1 diabetes mellitus (T1DM) skeletal muscle myosin heavy chain isoforms capillary network analysis muscle fibre composition 3D image analysis sladkorna bolezen tipa 1 (T1DM) skeletna mišica izoforme težke verige miozina analiza kapilarnih mrež sestava mišičnih vlaken analiza 3D slike true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-20 13:52:53 2024-06-20 13:53:00 2024-07-05 03:23:02 0000-00-00 00:00:00 2024 0 0 Str. 582-592 iss. 3 Vol. 24 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 611 2831-0896 10.17305/bb.2023.9843 171111427 bb-2023-9843.pdf bb-2023-9843.pdf 1 97658A35733C3A1768963F7336E50064 bd2f0287aadf60c6cc849ed7a3830d8a2155221e88deb41e873dc936e4f4c8e0 e521f12b-2efd-11ef-a73e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186526 https://bjbms.org/ojs/index.php/bjbms/article/view/9843 1 a627177d-2efb-11ef-a73e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186525 Medicinska fakulteta Biotehniška fakulteta 0 0 0