20.500.12556/RUL-158505 The mysteries of DNA preservation in bone a comparative study of petrous bones and metacarpal epiphyses using ATR-FTIR spectroscopy A comparative analysis of 26 petrous bones and epiphyses of metacarpals from the Second World War era revealed no significant differences in DNA yield or success in STR typing. This unexpected parity in DNA preservation between the petrous bone, a renowned source of endogenous DNA in skeletal remains, and the epiphyses of metacarpals, which are porous and susceptible to taphonomic changes, is surprising. In this study, we introduced ATR-FTIR spectroscopy as an approach to unravel the correlation between bone molecular structure and DNA preservation. Metacarpals and petrous bones with same taphonomic history were sampled and prepared for DNA analyses. While one portion of the sample was used for DNA analysis, the other underwent ATR-FTIR spectroscopic examination. The normalized spectra and FTIR indices between the epiphyses of metacarpals and petrous bones were compared. Because the taphonomic history of the remains used is relatively short and stable, the ATR-FTIR spectroscopy unveiled subtle structural differences between the two bone types. Petrous bones exhibited higher mineralization, whereas epiphyses contained more organic matter. The unexpected preservation of DNA in the epiphyses of metacarpals can likely be attributed to the presence of soft tissue remnants within the trabeculae. Here observed differences in the molecular structure of bones indicate there are different mechanisms enabling DNA preservation in skeletal tissues. ATR-FTIR spectroscopy DNA preservation metacarpals molecular structure petrous bone soft tissue remnants ATR-FTIR spektroskopija ohranjanje DNK metakarpalne kosti molekularna struktura petrorozna kost ostanki mehkih tkiv true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-14 12:00:42 2024-06-14 12:00:47 2024-06-29 03:50:24 0000-00-00 00:00:00 2024 0 0 8 str. art. 112076 Vol. 360 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61 1872-6283 10.1016/j.forsciint.2024.112076 198838019 1-s2.0-S0379073824001579-main.pdf 1-s2.0-S0379073824001579-main.pdf 1 F6751EBB9FE835F721E7709B4FA55CF6 34421425ea1ba4beb484dbb44e9bee1620196f10edc5de2a45c9803ad6b91e51 6802541f-2a35-11ef-86fa-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186170 https://www.sciencedirect.com/science/article/pii/S0379073824001579 0 faf50558-2a34-11ef-86fa-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186169 Filozofska fakulteta Medicinska fakulteta 0 0 0