20.500.12556/RUL-158452 Določanje parcialne molske toplotne kapacitete DNA oligonukleotidov Determination of parcial molar heat capacity of DNA oligonucleotides Za proteine obstajajo tako imenovane adicijske sheme, s pomočjo katerih je možno napovedati parcialno molsko toplotno kapaciteto denaturiranega (razvitega) stanja za poljuben protein na osnovi števila posameznih aminokislin v tem proteinu, kar je ključnega pomena za raziskave stabilnosti. V tem magistrskem delu sem se lotila raziskave aditivnosti parcialne toplotne kapacitete nukleotidov v DNK in razvoja enačbe, ki bi služila kot adicijska shema za oceno parcialne molske toplotne kapacitete denaturiranega (razvitega) stanja poljubnih enoverižnih DNK na podlagi merjenja parcialne molske toplotne kapacitete krajšim modelnim spojinam (DNK oligonukleotidov) z diferenčno dinamično kalorimetrijo. Določila sem prispevke za posamezne oligonukleotide (A, T, C, G) in tako parametrizirala enačbo za napoved parcialne molske toplotne kapacitete. Napovedane vrednosti se v grobem ujemajo s podatki, zato bi bilo potrebno parametre v enačbi izboljšati. Potencial za izboljšavo vidim v upoštevanju okolice oziroma najbližjih sosedov, ki imajo nezanemarljiv vpliv na prispevek posameznega nukleotida k parcialni molski toplotni kapaciteti. For proteins, so-called additive schemes exist, which allow predicting the partial molar heat capacity of the denatured (unfolded) state for any protein based on the number of individual amino acids in that protein, which is crucial for stability research. In this master's thesis, I undertook the investigation of the additivity of the partial heat capacity of nucleotides in DNA and the development of an equation that would serve as an additive scheme for estimating the partial molar heat capacity of denatured (unfolded) states of arbitrary single-stranded DNA based on measuring the partial molar heat capacity of shorter model compounds (DNA oligonucleotides) using differential scanning calorimetry (DSC). I determined the contributions for individual nucleotides (A, T, C, G) and as such parameterized the equation for predicting the partial molar heat capacity. The predicted values roughly match the data, indicating that the parameters in the equation need improvement. Potential for improvement lies in considering the surroundings or nearest neighbors, which have a significant influence on the contribution of individual nucleotides to the partial molar heat capacity. DSC DNK toplotna kapaciteta adicijske sheme DSC DNA heat capacity addition scheme true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-06-12 13:55:14 2024-06-12 13:55:22 2024-06-24 03:43:16 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 22809 198709251 Podlipnik_Ivana_-_Dolocanje_parcialne_molske_toplotne_kapacitete_DNA_oligonukleotidov.pdf Podlipnik_Ivana_-_Dolocanje_parcialne_molske_toplotne_kapacitete_DNA_oligonukleotidov.pdf 1 25D5EE40A3036498682A834A5E9D17C1 ba1408f14930a15b17aea7b7c4a3f216176cf05d57eb0651ad21759bb7139d2b ac199e9c-28b2-11ef-8ef4-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186085 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0