20.500.12556/RUL-158412 Mucoadhesive film for oral delivery of vaccines for protection of the respiratory tract The delivery of vaccines plays a pivotal role in influencing the strength and longevity of the immune response and controlling reactogenicity. Mucosal immunization, as compared to parenteral vaccination, could offer greater protection against respiratory infections while being less invasive. While oral vaccination has been presumed less effective and believed to target mainly the gastrointestinal tract, trans-buccal delivery using mucoadhesive films (MAF) may allow targeted delivery to the mucosa. Here we present an effective strategy for mucosal delivery of several vaccine platforms incorporated in MAF, including DNA plasmids, viral vectors, and lipid nanoparticles incorporating mRNA (mRNA/LNP). The mRNA/LNP vaccine formulation targeting SARS-CoV-2 as a proof of concept remained stable within MAF consisting of slowly releasing water-soluble polymers and an impermeable backing layer, facilitating enhanced penetration into the oral mucosa. This formulation elicited antibody and cellular responses comparable to the intramuscular injection, but also induced the production of mucosal IgAs, highlighting its efficacy, particularly for use as a booster vaccine and the potential advantage for protection against respiratory infections. The MAF vaccine preparation demonstrates significant advantages, such as efficient delivery, stability, and simple noninvasive administration with the potential to alleviate vaccine hesitancy. vaccine delivery mucoadhesive film mucosal immunity respiratory infection dostava cepiv mukoadhezivni film imuniteta sluznice okužba dihal sproščanje erozija imunski odziv dostavni sistemi cepiva true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-07 10:11:42 2024-06-07 10:11:47 2024-06-27 03:20:38 0000-00-00 00:00:00 2024 0 0 Str. 179-192 Vol. 371 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.371 0168-3659 10.1016/j.jconrel.2024.05.041 197189635 1-s2.0-S0168365924003262-main.pdf 1-s2.0-S0168365924003262-main.pdf 1 626B010FF79633B32924FE320B52DC01 1a63106af920e71e0d73a3595b5ed3c6912bd7cfdd3416086622ecfd85f3103e f30399c6-24a5-11ef-947b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186003 https://www.sciencedirect.com/science/article/pii/S0168365924003262 1 95fdaf36-24a5-11ef-947b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=186002 Fakulteta za farmacijo 0 0 0