20.500.12556/RUL-158319 DNA barcoding insufficiently identifies European wild bees (Hymenoptera, Anthophila) due to undefined species diversity, genus-specific barcoding gaps and database errors Recent declines in insect abundances, especially populations of wild pollinators, pose a threat to many natural and agricultural ecosystems. Traditional species monitoring relies on morphological character identification and is inadequate for efficient and standardized surveys. DNA barcoding has become a standard approach for molecular identification of organisms, aiming to overcome the shortcomings of traditional biodiversity monitoring. However, its efficacy depends on the completeness of reference databases. Large DNA barcoding efforts are (almost entirely) lacking in many European countries and such patchy data limit Europe-wide analyses of precisely how to apply DNA barcoding in wild bee identification. Here, we advance towards an effective molecular identification of European wild bees. We conducted a high-effort survey of wild bees at the junction of central and southern Europe and DNA barcoded all collected morphospecies. For global analyses, we complemented our DNA barcode dataset with all relevant European species and conducted global analyses of species delimitation, general and genus-specific barcoding gaps and examined the error rate in DNA data repositories. We found that (i) a sixth of all specimens from Slovenia could not be reliably identified, (ii) species delimitation methods show numerous systematic discrepancies, (iii) there is no general barcoding gap across all bees and (iv) the barcoding gap is genus specific, but only after curating for errors in DNA data repositories. Intense sampling and barcoding efforts in underrepresented regions and strict curation of DNA barcode repositories are needed to enhance the use of DNA barcoding for the identification of wild bees. insect pollinators wild bees DNA biodiversity databases insect decline pollinator species delimitation taxonomic impediment žuželke opraševalci divje čebele DNA biološka raznovrstnost zbirke podatkov true false true Angleški jezik Slovenski jezik Članek v reviji 2024-06-05 10:09:18 2024-06-05 10:09:19 2024-06-26 03:40:11 0000-00-00 00:00:00 2024 0 0 31 str. iss. 5, art. e13953 Vol. 24 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 595.799:57.088.7:631.527.82 1755-098X 10.1111/1755-0998.13953 191613699 Molecular_Ecology_Resources_-_2024_-_Janko_-_DNA_barcoding_insufficiently_identifies_European_wild_bees__Hymenoptera_.pdf Molecular_Ecology_Resources_-_2024_-_Janko_-_DNA_barcoding_insufficiently_identifies_European_wild_bees__Hymenoptera_.pdf 1 FB48DC1624A794A96775369DACC23440 858c7e55e2a29b84ae99c1d0760e885a567b48e4ee5638d10a3ab9eef31d81f5 9aec4099-2344-11ef-b111-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=185908 https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13953 0 24d91dcc-2313-11ef-b111-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=185885 Biotehniška fakulteta 0 0 0