20.500.12556/RUL-158240 Whole genome sequencing and molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from patients with bacteraemia in Slovenia Purpose Data on the molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from patients with bacteraemia in Slovenia are lacking. The aim of this study was to phenotypically and genotypically investigate 82 MRSA strains isolated from patients with bloodstream infections in central Slovenia between 2019 and 2022. Methods Whole-genome sequencing of selected strains was performed to characterize the strains based on sequence typing, antimicrobial resistance, toxin, and virulence factors genes. Results Most MRSA carried SCCmec II (63.4%), followed by SCCmec IV (34.1%) and SCCmec V (2.5%). A high proportion of strains belonging to the ST225 lineage (45.1%) was observed, followed by ST97 (18.3%), ST2883 (15.9%), ST22 (9.8%), ST5 (3.7%), and the ST1, ST398 and ST45 lineages (2.4% each). Sixteen different spa types were identified, predominantly ST225-t003 (31.7%), ST97-t359 (15.9%), and ST2883-t4336 (14.6%). None of the strains carried Panton-Valentine leukocidin, exfoliative toxins, or toxic shock toxin. All MRSA strains were susceptible to linezolid, rifampicin, vancomycin, teicoplanin, and trimethoprim-sulfamethoxazole. MRSA strains were resistant to erythromycin, clindamycin, tetracycline and gentamicin, with a frequency of 74.4%, 74.4%, 8.5%, and 1.2%, respectively. Conclusion This study demonstrates that bacteraemia in central Slovenia is caused by diverse MRSA lineages. Identification of newly emerged lineages should be followed in the future to detect changes in the molecular epidemiology of MRSA in our country. MRSA bloodstream infections whole genom sequencing Slovenia okužbe krvnega obtoka sekvenciranje celotnega genoma Slovenija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-05-30 15:38:05 2024-05-30 15:38:09 2024-06-18 03:42:57 0000-00-00 00:00:00 2024 0 0 Str. 969-977 iss. 5 Vol. 43 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-097:616.1 0934-9723 10.1007/s10096-024-04802-1 197289987 s10096-024-04802-1.pdf s10096-024-04802-1.pdf 1 DE61F612301BB9F5C185F0B51643C398 cdcc3a40144e266d5140cf0f657ffd4b6e1266f8f292f7fb2c1387d41f6728b5 193389c2-1e8a-11ef-b1e4-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=185764 https://link.springer.com/article/10.1007/s10096-024-04802-1 1 dbd1da81-1e89-11ef-b1e4-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=185763 Medicinska fakulteta 0 0 0