20.500.12556/RUL-156395 Joint identification and application of microsatellite markers in genetic diversity study of closely related species Teucrium montanum, T. capitatum and their natural hybrid Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo assembly was carried out resulting in 120 contigs for T. montanum and 1685 contigs for T. capitatum assembled. The Dig-up primers pipeline was used for SSR mining of both assemblies, applying different criteria that resulted in 112 SSR candidates for testing. A subset of 41 SSRs was selected and after two rounds of testing, twelve SSRs were developed and characterized. A total of 232 alleles were detected with 5 to 29 alleles per locus, based on the test sample. The genetic diversity analysis of three Teucrium taxa from a single location revealed a higher level of diversity in T. montanum than in T. capitatum with intermediate values for the hybrid species. The NeighborNet diagram and genetic structure analysis grouped the taxa into two separate clusters, the first of which consisted exclusively of T. montanum, while the other was composed of intermixed T. capitatum and T. × rohlenae individuals. The availability of SSR markers for two Teucrium taxa will allow in-depth analysis of genetic diversity and structure, as well as molecular identification of their putative hybrids in the future. Teucrium montanum Teucrim capitatum Teucrim x rohlenae genomic SSRs Lamiaceae molekularna genetika mikrosateliti molekularni markerji bioinformatika identifikacija Teucrium true false true Angleški jezik Slovenski jezik Članek v reviji 2024-05-23 10:04:48 2024-05-23 10:04:51 2024-05-24 03:56:52 0000-00-00 00:00:00 2024 0 0 12 str. iss. 4, art. 206 Vol. 16 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 1424-2818 10.3390/d16040206 196379395 diversity-16-00206-v2.pdf diversity-16-00206-v2.pdf 1 D3DDB987C5AF600014FB238D1F388BE7 0b6a2167c55c4bb3ec3e96abe1592c653d513b9594ec1d06ad4bd0af970f5570 7595f826-18db-11ef-850a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183835 https://www.mdpi.com/1424-2818/16/4/206 1 22c2fe56-18db-11ef-850a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183834 Biotehniška fakulteta 0 0 0