20.500.12556/RUL-156335 Biolayer interferometry and its applications in drug discovery and development Biolayer interferometry (BLI) is an optical ‘dip-and-read’ biosensor method for real-time, label-free analysis of biomolecular interactions. It can be used for kinetic analyses, analyte detection and quantitation with mid-to high throughput. Here, we review how BLI can support diverse activities in the broad field of drug design and development, ranging from fragment and compound library screening, structure-activity relationship and selectivity analyses of small-molecule drug leads, to cell line development for the production of biopharmaceutics, optimization and surveillance of bioprocess, and characterization and quality control of biologic drug substances. We discuss the strengths and drawbacks of BLI and compare it to other well-established methods and techniques. With advances in sensitivity, BLI has established itself as a robust and versatile tool for interrogating molecular interactions and structures, expanding from the world of biological molecules to small synthetic compounds. biosensor drug discovery library screening label-free analysis kinetics affinity biopharmaceutical development biosenzorji odkrivanje zdravil knjižnica presejalnih pregledov kinetika analiza brez nalepk afiniteta biofarmacevtski razvoj true false true Angleški jezik Slovenski jezik Članek v reviji 2024-05-20 15:39:39 2024-05-20 15:39:44 2024-05-21 03:46:45 0000-00-00 00:00:00 2024 0 0 13 str. art. 117741 Vol. 176 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.4:615.2 0165-9936 10.1016/j.trac.2024.117741 194830851 1-s2.0-S0165993624002243-main.pdf 1-s2.0-S0165993624002243-main.pdf 1 D68DA605BDF01C40C61BC3A43ECF0E10 a98260ba92c9ac397e4301dc754690af64ffa992eb92e0ec8a96f93dd6c9d627 b47eb9ad-16ae-11ef-b600-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183743 https://www.sciencedirect.com/science/article/pii/S0165993624002243 1 69ea2292-16ae-11ef-b600-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183742 Fakulteta za farmacijo 0 0 0