20.500.12556/RUL-156259 Platelet activation and blood extracellular vesicles the influence of venepuncture and short blood storage Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs. Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs. Results: In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype. Conclusions: Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known. blood blood plasma extracellular vesicles flow cytometry platelet activation kri krvna plazma zunajcelični vezikli pretočna citometrija aktivacija trombocitov true false true Angleški jezik Slovenski jezik Članek v reviji 2024-05-16 11:00:23 2024-05-16 11:00:27 2024-09-02 09:23:27 0000-00-00 00:00:00 2024 0 0 7 str. art. 102842 Vol. 106 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.1 1079-9796 10.1016/j.bcmd.2024.102842 192381699 1-s2.0-S1079979624000202-main.pdf 1-s2.0-S1079979624000202-main.pdf 1 B557466A25DD7E7B1A7FE91CF52B9903 849317221765072f5c86ce69b7327f6ee7743d308dc79e501316fd36368e7654 043fa878-1363-11ef-bd09-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183622 https://www.sciencedirect.com/science/article/pii/S1079979624000202 0 bcdff601-1362-11ef-bd09-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183621 Medicinska fakulteta Biotehniška fakulteta 0 0 0