20.500.12556/RUL-156196 Antibody association in solution cluster distributions and mechanisms Understanding factors that affect the clustering and association of antibodies molecules in solution is critical to their development as therapeutics. For 19 different monoclonal antibody (mAb) solutions, we measured the viscosities, the second virial coefficients, the Kirkwood-Buff integrals, and the cluster distributions of the antibody molecules as functions of protein concentration. Solutions were modeled using the statistical-physics Wertheim liquid-solution theory, representing antibodies as Y-shaped molecular structures of seven beads each. We found that high-viscosity solutions result from more antibody molecules per cluster. Multi-body properties such as viscosity are well predicted experimentally by the 2-body Kirkwood-Buff quantity, G22, but not by the second virial coefficient, B22, and well-predicted theoretically from the Wertheim protein–protein sticking energy. Weakly interacting antibodies are rate-limited by nucleation; strongly interacting ones by propagation. This approach gives a way to relate micro to macro properties of solutions of associating proteins. aggregation viscosity G22 modeling thermodynamic perturbation theory agregacija viskoznost G22 modeliranje termodinamska perturbacijska teorija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-05-13 14:45:29 2024-05-13 14:45:30 2024-05-14 03:49:33 0000-00-00 00:00:00 2024 0 0 9 str. no. 1, art. 2339582 Vol. 16 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.322 1942-0862 10.1080/19420862.2024.2339582 194752259 https://www.tandfonline.com/doi/full/10.1080/19420862.2024.2339582 1 b02c798d-1126-11ef-adc7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183515 RAZ_Brudar_Sandi_2024.pdf RAZ_Brudar_Sandi_2024.pdf 1 997238F8C3C90A4BD677B4FFD943B9CA 23038602764a64d8981f780a413bbed7912aa9da7fde62934a9df642aded29a2 662f7806-1129-11ef-adc7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183518 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0