20.500.12556/RUL-156123 Effects of phantom microstructure on their optical properties Significance Developing stable, robust, and affordable tissue-mimicking phantoms is a prerequisite for any new clinical application within biomedical optics. To this end, a thorough understanding of the phantom structure and optical properties is paramount. Aim We characterized the structural and optical properties of PlatSil SiliGlass phantoms using experimental and numerical approaches to examine the effects of phantom microstructure on their overall optical properties. Approach We employed scanning electron microscope (SEM), hyperspectral imaging (HSI), and spectroscopy in combination with Mie theory modeling and inverse Monte Carlo to investigate the relationship between phantom constituent and overall phantom optical properties. Results SEM revealed that microspheres had a broad range of sizes with average (13.47±5.98)μm and were also aggregated, which may affect overall optical properties and warrants careful preparation to minimize these effects. Spectroscopy was used to measure pigment and SiliGlass absorption coefficient in the VIS-NIR range. Size distribution was used to calculate scattering coefficients and observe the impact of phantom microstructure on scattering properties. The results were surmised in an inverse problem solution that enabled absolute determination of component volume fractions that agree with values obtained during preparation and explained experimentally observed spectral features. HSI microscopy revealed pronounced single-scattering effects that agree with single-scattering events. Conclusions We show that knowledge of phantom microstructure enables absolute measurements of phantom constitution without prior calibration. Further, we show a connection across different length scales where knowledge of precise phantom component constitution can help understand macroscopically observable optical properties. biomedical optics hyperspectral imaging spectroscopy microscopy tissue phantoms biomedicinska optika hiperspektralno slikanje spektroskopija mikroskopija tkivni fantomi true false true Angleški jezik Slovenski jezik Članek v reviji 2024-05-09 12:30:45 2024-05-09 12:30:46 2024-05-10 03:45:54 0000-00-00 00:00:00 2024 0 0 Str. 093502-1-093502-16 iss. 9 Vol. 29 2024 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 2024-05-06 535 1083-3668 10.1117/1.JBO.29.9.093502 194902787 093502_1.pdf 093502_1.pdf 1 E04B2EDC81F42D8C20619A7C8A48434E 787bf7b369bd96c182496942d85ab0c60d83d6a953871c820241236ff8a13ab7 9fed1af5-0def-11ef-b552-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183402 https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-29/issue-09/093502/Effects-of-phantom-microstructure-on-their-optical-properties/10.1117/1.JBO.29.9.093502.full 0 d4faaff4-0def-11ef-b552-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183403 Fakulteta za matematiko in fiziko 0 0 0