20.500.12556/RUL-155985 Morfometrična analiza filogeografskih skupin jamskega hrošča drobnovratnika (Leptodirus hochenwartii) Morphometric analysis of phylogroups of the cave beetle Leptodirus hochenwartii (Coleoptera: Leiodidae) Drobnovratnik (Leptodirus hochenwartii Schmidt, 1832) je ena izmed ikoničnih podzemeljskih živali. Kljub temu taksonomija vrste ostaja zapletena in nerazrešena. Zato smo z uporabo klasične in geometrijske morfometrije skušali opredeliti morfološke razlike med predhodno ugotovljenimi šestimi filogenetskimi skupinami. Analizo smo izvedli na 170 primerkih iz celotnega areala vrste. Vse primerke smo fotografirali pod stereomikroskopom. Fotografije smo uporabili kot osnovo za digitalne meritve dolžin, ki smo jih uporabili za analizo varianc (ANOVA) in linearno diskriminantno analizo (LDA). S slednjo smo na podlagi več znakov dosegli relativno visoko stopnjo razvrščanja v skladu s filogenetskimi skupinami. Iz fotografij primerkov smo z geometrijsko morfometrijo analizirali obliko pronotuma in desne pokrovke ter razlike med njimi izrazili z Prokrustovimi distancami. Na teh vrednostih smo uporabili analizo glavnih komponent (PCA) ter izračunali statistično značilnost razlik med skupinami z multivariatno analizo varianc (MANOVA). Razlike smo zaznali tako med oblikami pronotuma kot desnimi pokrovkami, a je bila oblika pronotuma veliko bolj informativna za ločevanje filogenetskih skupin. Naši rezultati predstavljajo izhodišče za taksonomsko revizijo rodu Leptodirus, ker tudi z morfološkimi podatki potrjujejo vrstni status filogenetskih skupin. Slenderneck beetle (Leptodirus hochenwartii Schmidt, 1832 is one of the iconic subterranean animals. Nevertheless, the taxonomy of the species remains complex and unresolved. Therefore, using classical and geometric morphometrics, we attempted to identify morphological differences between six predefined phylogenetic groups of the species. We conducted the analysis on 170 individuals from the entire species range. All specimens were photographed under a stereomicroscope. These photographs served as the basis for linear digital measurements, which were then used for analysis of variance (ANOVA) and linear discriminant analysis (LDA). Through the latter, based on multiple measurements we achieved a relatively high rate of correctly identified individuals. Additionally, differences between groups were tested with geometric morphometry. From the photographs, we obtained coordinates of the shape of pronotum and right elytron and performed a Procrustes analysis on them. Using these data, we conducted principal component analysis (PCA) and calculated the statistical significance of differences between groups with multivariate analysis of variance (MANOVA). Differences were detected both in the shape of the pronotum and the shape of the right elytron with the pronotum shape proving to be much more informative in distinguishing phylogenetic groups. Our results will serve as a basis for a taxonomic revision of the genus Leptodirus, because they further confirm that the phylogeographic groups are independent taxa at the species level. Leptodirus hochenwartii geometrijska morfometrija kriptične vrste Dinarski kras filogenija speleobiologija Leptodirus hochenwartii geometric morphometry cryptic species Dinaric karst phylogeny speleobiology true false false [D. Milošević] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-04-26 07:16:04 2024-04-26 07:16:11 2024-04-29 08:16:15 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 595.76:572.7(043.2) 236492 194149635 39525.pdf 39525.pdf 1 7657C4E423BB907DA67680593E57EB72 719c700d0feb91aff38aa9f1fec9cd70742240172daa9de4a8ceb908fe1045f5 18207d07-038c-11ef-9119-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183181 Biotehniška fakulteta 0 0 0