20.500.12556/RUL-155873 The efficiency of cbd production using grafted Cannabis sativa L. plants is highly dependent on the type of rootstock a study The global cannabis market is continuously expanding and as a result, the cannabis industry demands new and improved agronomic cultivation practices to increase production efficiency of cannabidiol (CBD), which is valued for its therapeutic benefits. This study investigates the influence of three rootstock types on the survival rate, morphological parameters, and biochemical composition of cannabis: potentially dwarfing rootstocks (PDR), potentially vigorous rootstocks (PVR), and seedlings-as-rootstocks (SAR). Rootstocks were used for grafting two scion genotypes: ‘ScionII’ = chemotype II of industrial hemp, and ‘ScionIII’ = chemotype III of high CBD accumulating variety. Contrary to expectations, PVR and SAR did not outperform PDR on most of the measured variables. SAR showed the highest survival rate of the grafted cannabis plants (40–70%). The rootstock type had a statistically significant influence only on the bud compactness index in ‘ScionII’, with PDR being particularly noticeable. A comparative analysis of the ‘rootstock/scion’ combinations with their controls (non-grafted scions) revealed grafting’s substantial improvement in most traits. Specifically, PDR increased CBD content by 27% in ‘ScionIII’, inflorescence yield and CBD yield per plant increased by 71% and 84%, respectively, when SAR was used in ‘ScionII’. SAR showed to be the most effective rootstock type for CBD production. Our findings suggest grafting as a promising technique for optimizing cannabis’s agronomic and medicinal potential, highlighting the necessity for further research on its underlying mechanisms to refine production efficiency and quality. Cannabis sativa L. cannabinoids grafting rootstock scion konoplja kanabinoidi CBD cepljenje podlage kemična sestava true false true Angleški jezik Slovenski jezik Članek v reviji 2024-04-23 12:35:21 2024-04-23 12:35:27 2024-04-24 04:20:22 0000-00-00 00:00:00 2024 0 0 24 str. iss. 8, art. 1117 Vol. 13 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633 2223-7747 10.3390/plants13081117 193537795 plants-13-01117.pdf plants-13-01117.pdf 1 8576A14433C905E3D38457E9528E85D9 68827f063d1960492d42d4c3bbb0b70447d33609fe675c0d2e059031aa49c686 dd435d4e-015d-11ef-9b79-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182992 https://www.mdpi.com/2223-7747/13/8/1117 1 34125541-015d-11ef-9b79-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182990 Biotehniška fakulteta Zdravstvena fakulteta 0 0 0