20.500.12556/RUL-155829 Recommendations from the COST action CA17116 (SPRINT) for the standardization of perinatal derivative preparation and in vitro testing Many preclinical studies have shown that birth-associated tissues, cells and their secreted factors, otherwise known as perinatal derivatives (PnD), possess various biological properties that make them suitable therapeutic candidates for the treatment of numerous pathological conditions. Nevertheless, in the field of PnD research, there is a lack of critical evaluation of the PnD standardization process: from preparation to in vitro testing, an issue that may ultimately delay clinical translation. In this paper, we present the PnD e-questionnaire developed to assess the current state of the art of methods used in the published literature for the procurement, isolation, culturing preservation and characterization of PnD in vitro. Furthermore, we also propose a consensus for the scientific community on the minimal criteria that should be reported to facilitate standardization, reproducibility and transparency of data in PnD research. Lastly, based on the data from the PnD e-questionnaire, we recommend to provide adequate information on the characterization of the PnD. The PnD e-questionnaire is now freely available to the scientific community in order to guide researchers on the minimal criteria that should be clearly reported in their manuscripts. This review is a collaborative effort from the COST SPRINT action (CA17116), which aims to guide future research to facilitate the translation of basic research findings on PnD into clinical practice. perinatal derivative preparation standardization in vitro testing recommendations SPRINT perinatal derivatives CA17116 PnD e-questionnaire perinatalni pripravek derivata standardizacija testiranje in vitro priporočila SPRINT true false true Angleški jezik Slovenski jezik Članek v reviji 2024-04-22 09:20:04 2024-04-22 09:20:12 2024-04-26 04:07:03 0000-00-00 00:00:00 2023 0 0 12 str. art. 1258753 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 61:576 2296-4185 10.3389/fbioe.2023.1258753 173352963 fbioe-11-1258753.pdf fbioe-11-1258753.pdf 1 665944AC2CDCC4472C7F5F22A53FAD61 d0978dc729b7d5a11f70a2f1194521899501576a8dd541e5451d765a1dde0d88 70946c96-02f7-11ef-a27e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183171 https://www.frontiersin.org/articles/10.3389/fbioe.2023.1258753/full 1 c2b87ce3-0078-11ef-9477-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182934 Medicinska fakulteta 0 0 0