20.500.12556/RUL-155724 Genetska raznolikost virusa Usutu v Sloveniji Genetic diversity of Usutu virus in Slovenia Virus Usutu je RNA virus, ki je bil v Evropo prenesen iz Afrike konec 20. stoletja. V Evropi vse pogosteje povzroča epidemije pri živalih, občasno okužbe z virusom dokažemo tudi pri ljudeh. Kot v predhodnih raziskavah tudi mi ugotavljamo, da virus v Sloveniji kroži. Protitelesa so že dokazali pri različnih živalskih vrstah v ljubljanskem živalskem vrtu, zavedeni pa so tudi z virusom Usutu (USUV) povezani pogini divjih ptic leta 2018. Leta 2018 smo ujeli dve USUV pozitivni skupini komarjev rodu Culex, leta 2019 kar šest in leta 2020 eno skupino. V sezoni 2021 nismo ujeli nobenega USUV pozitivnega komarja. Naša raziskava je prva raziskava genetske raznolikosti slovenskih sevov USUV. Devet sevov je bilo dokazanih v komarjih ulovljenih v različnih slovenskih krajih med leti 2018 in 2020. Celotne genome osmih sevov smo amplikonsko sekvenirali s tehnologijo Illumina in naredili filogenetsko analizo.Vseh osem sevov se uvršča v linijo Evropa 2, ki je takrat in še vedno prevladuje tudi v sosednjih državah. V Sloveniji smo dokazali dve filogenetski skupini virusa. Filogenetska primerjava s sevi iz sosednjih držav nakazuje na večkraten vnos virusa v Slovenijo predvsem iz Italije, verjetno pa tudi iz Avstrije in Madžarske. Možno je, da je virus na območju Slovenije prezimil in postaja endemičen. Naše raziskave kroženja in spreminjanja virusa bi bile natančnejše, če bi bilo javno dostopnih več sekvenciranih celotnih genomov USUV iz Evrope. Potreben je multidisciplinaren pristop, ki bi vključeval virologe, zdravstveno osebje, ornitologe in entomologe ter tesnejše sodelovanje med zdravstvenim, kmetijskim in okoljskim sektorjem, da bi lažje opredelili glavne dejavnike tveganja z namenom izvajanja ustreznih metod spremljanja in preprečevanja USUV. Usutu virus is an RNA virus which was introduced into Europe from Africa at the end of the 20th century. It has been causing increasingly more zootics in Europe, and infections have been diagnosed in humans. As in previous studies, we also noted that the virus is circulating in Slovenia. Antibodies have already been detected in various animal species of a zoological garden in Ljubljana and USUV associated deaths of wild birds have been reported in 2018. Two Culex mosquito pools sampled in 2018, six in 2019 and one in 2020 tested positive for USUV. In the season of 2021 no USUV positive mosquito pools were sampled. Our study is the first to investigate the genetic diversity of Slovenian USUV strains. Nine strains were confirmed in mosquitoes sampled at different Slovenian sites between 2018 and 2020. The complete genomes of eight strains were amplicon sequenced using Illumina technology and phylogenetic analysis was performed. All eight strains belong to the Europe 2 lineage, which all the while has been and continues to prevail in neighbouring countries. Two phylogenetic groups were demonstrated in Slovenia. Phylogenetic comparison with strains from neighbouring countries suggests multiple introductions of the virus into Slovenia, mainly from Italy, but probably also from Austria and Hungary. It is possible that the virus has overwintered in Slovenia and is becoming endemic. There are many data gaps on the epidemiology of USUV. Our research on virus circulation and changing could be more accurate, if more USUV complete genome sequences from Europe were publicly available. A multidisciplinary approach involving virologists, health personnel, ornithologists and entomologists is needed, as well as closer collaboration between the health, agricultural and environmental sectors to better identify the main risk factors in order to implement appropriate USUV monitoring and prevention methods. virus Usutu invazivne tujerodne vrste komarji Culex pipiens genetska raznolikost molekularna diagnostika sekvenciranje NGS Usutu virus invasive non-native species mosquitoes Culex pipiens genetic diversity molecular diagnostics sequencing NGS true false false [A. Koletnik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-04-13 07:15:07 2024-04-13 07:15:20 2024-04-17 13:15:04 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 578.7:579.77:578.833.2:57.083.2 236434 192866307 39082.pdf 39082.pdf 1 2F9D6BBCB8F557F416BCA7F3A2B41262 d5ec5c0408530190acdad2b9b01e2389c669b5a3202b35c8a7cff64b1eacfb94 d3a41ef5-f954-11ee-950c-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182782 Biotehniška fakulteta 0 0 0