20.500.12556/RUL-155611 Cationised fibre-based cellulose multi-layer membranes for sterile and high-flow bacteria retention and inactivation Low-cost, readily available, or even disposable membranes in water purification or downstream biopharma processes are becoming attractive alternatives to expensive polymeric columns or filters. In this article, the potential of microfiltration membranes prepared from differently orientated viscose fibre slivers, infused with ultrafine quaternised (qCNF) and amino-hydrophobised (aCNF) cellulose nanofibrils, were investigated for capturing and deactivating the bacteria from water during vacuum filtration. The morphology and capturing mechanism of the single- and multi-layer structured membranes were evaluated using microscopic imaging and colloidal particles. They were assessed for antibacterial efficacy and the retention of selected bacterial species (Escherichia coli, Staphylococcus aureus, Micrococcus luteus), differing in the cell envelope structure, hydrodynamic biovolume (shape and size) and their clustering. The aCNF increased biocidal efficacy significantly when compared to qCNF-integrated membrane, although the latter retained bacteria equally effectively by a thicker multi-layer structured membrane. The retention of bacterial cells occurred through electrostatic and hydrophobic interactions, as well as via interfibrous pore diffusion, depending on their physicochemical properties. For all bacterial strains, the highest retention (up to 100% or log 6 reduction) at >50 L/h∗bar∗m$^2$ flow rate was achieved with a 4-layer gradient-structured membrane containing different aCNF content, thereby matching the performance of industrial polymeric filters used for removing bacteria. fibrous membrane cationised cellulose nanofibrils amino-hydrophobised cellulose nanofibrils antibacterial activity multi-layer structure flux bacteria retention fibrozne membrane kationizirani celulozni nanofibrili amino-hidrofobizirani celulozni nanofibrili protibakterijska aktivnost večplastna struktura tok zadrževanje bakterij true false true Angleški jezik Slovenski jezik Članek v reviji 2024-04-08 12:28:46 2024-04-08 12:28:48 2024-04-09 03:46:02 0000-00-00 00:00:00 2023 0 0 23 str. iss. 3, art. 284 Vol. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577:620.3 2077-0375 10.3390/membranes13030284 144515331 membranes-13-00284-v2.pdf membranes-13-00284-v2.pdf 1 65A81CAC8D3A357A6EC2CA709D12C4D4 ad093dade4c9c8ebc6e15e258c017a2db1ddf3f04b52a7412efa0283c2fa04ce 0bc73b5d-f593-11ee-937a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182589 https://www.mdpi.com/2077-0375/13/3/284 0 f2c76703-f592-11ee-937a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182588 Biotehniška fakulteta 0 0 0