20.500.12556/RUL-155482 Association between sperm morphology and altered sperm microRNA expression Evaluation of male infertility has been based on semen analysis for years. As this method can be subjective at times, there is a scientific tendency to discover stable and quantifiable biomarkers. This study included 28 couples who underwent an in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycle. The couples were assigned into two groups, according to sperm morphology. Couples where the males were normozoospermic were placed in the control group (15 participants), while couples where males had teratozoospermia were placed in the study group (13 participants). Thirteen candidate miRNAs were selected for qPCR analysis, based on our literature search. We determined significant under-expression of nine miRNAs (miR-10a-5p/-15b-5p/-26a-5p/-34b-3p/-122-5p/-125b-5p/-191-5p/-296-5p and let-7a-5p) in spermatozoa from patients with teratozoospermia compared to the controls, whereas expression levels of four miRNAs (miR-92a-3p/-93-3p/-99b-5p/-328-3p) did not significantly differ between the study and control groups. The expression levels of all 13 included miRNAs were significantly positively correlated with each other and significantly positively associated with spermatozoa morphology, excluding miR-99b-5p. There were no other significant associations between miRNA expression and sperm quality parameters. Only expression levels of miR-99b-5p were significantly positively correlated with good-quality day 3 embryo rate (ρ = 0.546; p = 0.003), while other variables of the IVF/ICSI cycle outcome showed no significant associations with miRNA expression profiles. This is one of the rare studies providing an insight directly into miRNA profiles in regard to sperm morphology. We identified nine miRNAs that could serve as biomarkers of spermatozoa quality in regard to teratozoospermia. sperm morphology microRNA male infertility teratozoospermia morfologija semenčic mikroRNA moška neplodnost true false true Angleški jezik Slovenski jezik Članek v reviji 2024-04-04 11:11:10 2024-04-04 11:11:15 2024-04-05 03:46:23 0000-00-00 00:00:00 2022 0 0 14 str. iss. 11, art. 1671 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.6 2079-7737 10.3390/biology11111671 130006787 biology-11-01671.pdf biology-11-01671.pdf 1 406F640E8953271533F05C1338E064E8 8154a2236d6d9fa7f0c38455ee0d413f8a1c1127e1e8e12a394e4ade24a61a57 aeaba0e8-f263-11ee-87dd-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182400 https://www.mdpi.com/2079-7737/11/11/1671 1 4a0fce0a-f263-11ee-87dd-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182399 Medicinska fakulteta 0 0 0