20.500.12556/RUL-155466 Inflammatory, oxidative stress and small cellular particle response in HUVEC induced by debris from endoprosthesis processing We studied inflammatory and oxidative stress-related parameters and cytotoxic response of human umbilical vein endothelial cells (HUVEC) to a 24 h treatment with milled particles simulating debris involved in sandblasting of orthopedic implants (OI). We used different abrasives (corundum—(—Al$_2$O$_{3}$), used corundum retrieved from removed OI (u. Al$_2$O$_{3}$), and zirconia/silica composite (ZrO$_2$/SiO$_{2}$). Morphological changes were observed by scanning electron microscopy (SEM). Concentration of Interleukins IL-6 and IL-1$\beta$ and Tumor Necrosis Factor $\alpha$ (TNF)-$\alpha$ was assessed by enzyme-linked immunosorbent assay (ELISA). Activity of Cholinesterase (ChE) and Glutathione S-transferase (GST) was measured by spectrophotometry. Reactive oxygen species (ROS), lipid droplets (LD) and apoptosis were measured by flow cytometry (FCM). Detachment of the cells from glass and budding of the cell membrane did not differ in the treated and untreated control cells. Increased concentration of IL-1$\beta$ and of IL-6 was found after treatment with all tested particle types, indicating inflammatory response of the treated cells. Increased ChE activity was found after treatment with u. Al$_2$O$_{3}$ and ZrO$_2$/SiO$_{2}$. Increased GST activity was found after treatment with ZrO$_2$/SiO$_{2}$. Increased LD quantity but not ROS quantity was found after treatment with u. Al$_2$O$_{3}$. No cytotoxicity was detected after treatment with u. Al$_2$O$_{3}$. The tested materials in concentrations added to in vitro cell lines were found non-toxic but bioactive and therefore prone to induce a response of the human body to OI. endoprosthesis failure corundum cytokines reactive oxygen species lipid droplets true false false Angleški jezik Angleški jezik Članek v reviji 2024-04-03 14:28:42 2024-04-03 14:28:43 2024-04-04 03:52:39 0000-00-00 00:00:00 2023 0 0 Str. 1-17 iss. 9 Vol. 16 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 691.714.018.8 1996-1944 10.3390/ma16093287 150454019 materials-16-03287-v2_(1).pdf materials-16-03287-v2_(1).pdf 1 4BAD9AB151C4A41D8555DB9CDD53D1A9 828e934aef27180eaef31135ff8fe2233adb8bfd504a12cbfdaa52c06600121f 490033aa-f1b7-11ee-853b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182372 https://www.mdpi.com/1996-1944/16/9/3287 1 b5b2ebab-f1b5-11ee-853b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182369 Fakulteta za elektrotehniko Zdravstvena fakulteta Biotehniška fakulteta Fakulteta za farmacijo Medicinska fakulteta 0 0 0