20.500.12556/RUL-155360 Broad host range may be a key to long-term persistence of bacteriophages infecting intestinal Bacteroidaceae species The longitudinal studies have found that the human gut microbiota is stable over time with some major bacterial lineages or even strains persisting for years. This was recently extended to gut bacteriophages using the metagenomic data. Here, we focused on cultivation of the major Bacteroidetes of human gut, the Bacteroides and Phocaeicola strains, and their bacteriophages from two healthy donors. The persistence of Bacteroides and Phocaeicola species and strains was confirmed. We isolated 28 genetically different phages grouped into seven distinct clusters, two of these were new. Moreover, the bacteriophages from several groups, although being genetically quite homogeneous, had the ability to infect the strains belonging to different species isolated from several sampling time-points and different donors. We propose that the ability to infect several host species, which differ in their nutritional niches, may promote long-term persistence of dominant gut bacteriophage groups. bacteriophages microbiome phage biology črevesna mikrobiota Bacteroidaceae bakteriofagi true false true Angleški jezik Slovenski jezik Članek v reviji 2024-03-27 15:08:42 2024-03-27 15:08:43 2024-03-28 03:55:25 0000-00-00 00:00:00 2022 0 0 11 str. Vol. 12, art. 21098 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.61:578.347:579.844.1 2045-2322 10.1038/s41598-022-25636-x 133368323 s41598-022-25636-x.pdf s41598-022-25636-x.pdf 1 5C938E408FA0A82D82AEE24368FDD9AB 92f4c0199c1f95a5a47d6ff2b86bded3d0f015b15fa737afb1c3a73dd649c3c3 d2b121db-ec43-11ee-aa14-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182209 https://www.nature.com/articles/s41598-022-25636-x 0 b0b62c5d-ec43-11ee-aa14-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182208 Biotehniška fakulteta 0 0 0