20.500.12556/RUL-155264 Exploring the potential of cold plasma treatment followed by zinc-priming for biofortification of buckwheat sprouts Increasing the concentration of an element in edible produce (i.e., biofortification) can mitigate the element deficiency in humans. Sprouts are small but popular part of healthy diets providing vitamins and essential elements throughout the year. Element composition of sprouts can easily be amended, e.g., by soaking the grains in element-rich solution before germination (grain-priming). In addition, pre-treatment of grains to improve element translocation from the solution into the grain may further enhance the element concentration in the sprout. Cold plasma technique could provide such solution, as it increases wettability and water uptake of grains. Grains of common buckwheat (Fogopyrum esculentum Moench) were pre-treated/untreated with cold plasma and soaked in ZnCl$_2$ solution/pure water. Germination tests, α-amylase activity, grain hydrophilic properties and water uptake were assessed. Element composition of grain tissues and of sprouts was assessed by micro-particle-induced-X-ray emission and X-ray fluorescence spectroscopy, respectively. Grain-priming increased Zn concentration in shoots of common buckwheat sprouts more than five-times, namely from 79 to 423 mg Zn kg$^{−1}$ dry weight. Cold plasma treatment increased grain wettability and water uptake into the grain. However, cold plasma pre-treatment followed by grain-priming with ZnCl$_2$ did not increase Zn concentration in different grain tissues or in the sprouts more than the priming alone, but rather decreased the Zn concentration in sprout shoots (average ± standard error: 216 ± 6.13 and 174 ± 7.57 mg Zn kg$^{−1}$ dry weight, respectively). When the fresh weight portion of whole sprouts (i.e., of roots and shoots) was considered, comparable average requirements of Zn, namely 24.5 % and 35 % for adult men and women would be satisfied by consuming cold plasma pre-treated and not pre-treated grains. Potential advantages of cold plasma pre-treatment need to be tested further, mainly to optimize the duration of soaking required to produce Zn-enriched sprouts. common buckwheat Fagopyrum esculentum non-thermal plasma zinc distribution grain tissues Fagopyrum esculentum navadna ajda true false true Angleški jezik Slovenski jezik Članek v reviji 2024-03-22 12:12:56 2024-03-22 12:13:01 2024-03-23 04:15:02 0000-00-00 00:00:00 2023 0 0 12 str. Vol. 10, art. 1151101 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 581 2296-861X 10.3389/fnut.2023.1151101 151072003 fnut-10-1151101.pdf fnut-10-1151101.pdf 1 7A61619BE2AEBA227041EDA19A2D5D90 d41c9d5d1f833b938fe711a430c3a6365bbaac30128a7f62ae50cd0c99c89d5a 1ce8399a-e83e-11ee-b815-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182078 https://www.frontiersin.org/articles/10.3389/fnut.2023.1151101/full 1 26f2a804-e83d-11ee-b815-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182076 Biotehniška fakulteta 0 0 0