20.500.12556/RUL-155038 Morfološka in molekularna identifikacija jamskih mikroalg za potencialno uporabo v biotehnologiji Morphological and molecular identification of cave microalgae for potential use in biotechnology Ekstremofilnim mikroalgam preživetje omogoča njihova neverjetna sposobnost prilaganja na dane razmere ter evolucijska pot, ki je vodila v razvoj izjemno fiziološko in morfološko različnih organizmov. Najdemo jih tudi v jamah, kjer so fototrofni organizmi bolj redko naseljeni. Primera takih jam sta Postojnska jama in Škocjanske jame v Sloveniji. V okvire te naloge smo preučevali vrstno sestavo teh mikroorganizmov preko taksonomske analize ter primerjali razlike v raznovrstnosti ob vzorčenju in po treh letih gojenja v laboratoriju. Preučevali smo tudi njihove biokemijske značilnosti z infrardečo spektroskopijo s Fourierevo transformacijo (FTIR). Ugotovili smo, da se je raznovrstnost izrazito zmanjšala tako pri prokariontskih kot tudi pri evkariontskih organizmih. Delež evkariontskih alg in cianobakterij znotraj posameznega vzorca je bil v 2020 približno enak, v letu 2023 pa prevladujejo evkarionti. FTIR spektri mikroalg so se minimalno razlikovali med sabo, tako lokacijsko kot tudi časovno, iz česar lahko sklepamo, da imajo jamske mikroalge značilen FTIR profil. Mikroalge imajo izjemen biotehnološki pomen, saj so poceni tovarne za pridelavo spojin, ki imajo visoko vrednost v prehranski, kozmetični in farmacevtski industriji ter na področju biomedicine. Obenem nam omogočajo zmanjšanje emisij atmosferskega CO2, medtem ko se istočasno uporabljajo za proizvodnjo biogoriva ali čiščenje onesnaženih voda. Skratka, možnosti, ki jih ponujajo, so res številne in perspektivne za ustvarjanje bolj zelene prihodnosti. Extremophilic microalgae survive thanks to their incredible ability to adapt to given conditions and an evolutionary path that has led to the development of extremely physiologically and morphologically diverse organisms. They are also found in caves, where phototrophic organisms are less commonly inhabited. Examples of such caves are the Postojna Cave and the Škocjan Caves in Slovenia. In this study, we examined the species composition of these microorganisms through taxonomic analysis and compared differences in diversity between sampling and after three years of cultivation in the laboratory. We also investigated their biochemical characteristics using Fourier-transform infrared spectroscopy (FTIR). We found that diversity significantly decreased in both prokaryotic and eukaryotic organisms. The percentage of microalgae in the samples increased, with a greater increase observed in eukaryotes. FTIR spectra of microalgae minimally differed among themselves, both locationally and temporally, indicating that cave microalgae have a characteristic FTIR profile. Microalgae have significant biotechnological importance as they serve as inexpensive factories for producing compounds that are highly valued in the food, cosmetic, pharmaceutical industries, and in biomedicine. Additionally, they enable us to reduce atmospheric CO2 emissions while simultaneously being used for biofuel production or cleaning polluted waters. In short, the opportunities they offer are numerous and promising for creating a greener future. Mikroalge biotehnologija sekvenciranje genoma taksonomska analiza FTIR Microalgae biotechnology genomic sequencing taxonomic analysis FTIR true false false [B. Domevščik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-03-16 07:15:07 2024-03-16 07:15:19 2024-03-18 08:30:28 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 606:582.3(043.2) 236359 189198339 39140.pdf 39140.pdf 1 308279146DA980BA4DCFA691A99ED600 a2c6e63f628652f696b102a23286ef9a9c33b9ac99581552ecfb4392b50ae879 8f877926-e35c-11ee-87c3-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181764 Biotehniška fakulteta 0 0 0