20.500.12556/RUL-154949 A synergistic anti-bacterial and anti-adhesion activity of tea tree (Melaleuca alternifolia) and lemon eucalyptus tree (Eucalyptus citriodora Hook) essential oils on Legionella pneumophila Legionella pneumophila is a Gram-negative bacterial pathogen that colonizes natural and artificial water systems and has the ability to form a biofilm. The biofilm protects L. pneumophila from various environmental factors and makes it more resistant to chlorine-based disinfectants. This study investigated the anti-bacterial properties of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel) oil and lemon eucalyptus tree (Eucalyptus citriodora Hook) essential oils (EOs) and their synergistic, additive inhibitory and anti-adhesive effects against L. pneumophila biofilm formation on polystyrene. The minimum effective concentration (MEC) for tea tree is 12.8 mg ml$^{−1}$ and for lemon eucalyptus tree EO 6.4 mg ml$^{−1}$. In the checkerboard assay, different combinations of these two EO show synergistic and additive anti-microbial activity. The minimum anti-adhesive concentration (MAC) for tea tree is 12.8 mg ml$^{−1}$ and for lemon eucalyptus tree EO 6.4 mg ml$^{−1}$. A combination of 3.2 mg ml$^{−1}$ tea tree EO and 0.8 mg ml$^{−1}$ lemon eucalyptus tree EO showed the strongest anti-adhesive effect against L. pneumophila on polystyrene. The tested oils and their combination showed intriguing potential to inhibit L. pneumophila biofilm formation. anti-Legionella activity anti-adhesion mass spectrometry essential oils checkerboard assay true false true Angleški jezik Ni določen Članek v reviji 2024-03-11 14:08:57 2024-03-11 14:08:59 2024-05-08 09:53:47 0000-00-00 00:00:00 2024 0 0 Str. 54-63 no. 1 Vol. 40 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 579.2:543.51:614 0892-7014 10.1080/08927014.2024.2310482 188405251 A_synergistic_anti-bacterial_and_anti-adhesion_activity_of_tea_tree__Melaleuca_alternifolia__and_lemon_eucalyptus_tree__Eucalyptus_cit.pdf A_synergistic_anti-bacterial_and_anti-adhesion_activity_of_tea_tree__Melaleuca_alternifolia__and_lemon_eucalyptus_tree__Eucalyptus_cit.pdf 1 C1DEC98631BAAE4D115DCFC773BBA2DD cef41b3f8af94ff89c59549707aa862a6d8ad1a0bf981840207f79b3dfe9a000 2c523ff4-0c4e-11ef-a791-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=183290 https://www.tandfonline.com/doi/full/10.1080/08927014.2024.2310482 0 0b333e33-dfa9-11ee-a66a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181619 Zdravstvena fakulteta 0 0 0