20.500.12556/RUL-154929 Vpliv enantiomerov dovoljenih in nedovoljenih drog na zarodke cebric (Danio rerio) Effects of enantiomers of licit and illicit drugs on zebrafish (Danio rerio) embryos V okolju so zaradi človekovega delovanja vse bolj prisotna onesnažila, ki imajo lahko kvarne učinke na organizme in združbe ter posredno tudi na človeka. V iztokih čistilnih naprav lahko zaznamo ekotoksikološko relevantne koncentracije tovrstnih spojin, med katerimi so tudi ostanki prepovedanih drog, ki prek njih vstopajo v površinske in podzemne vode, od tam pa tudi v pitno vodo. Namen tega magistrskega dela je bil testirati ekotoksičnost prepovedanih drog amfetamina, metamfetamina in MDMA (ekstazi), natančneje njihovih čistih enantiomerov in racematov, za ribe cebrice (Danio rerio). Ekotoksikološko testiranje smo izvajali po smernicah OECD TG 236 (OECD, 2013) za izvedbo testiranja akutne strupenosti na ribjih zarodkih. Kot nadgradnjo te metode smo izvedli tudi preliminarno študijo gibanja larv po 96-urni izpostavitvi testiranim spojinam. Pri nobeni od testiranih spojin pri koncentracijah med 0,2 in 8,6 mg/L po 96-urni izpostavitvi v primerjavi z negativno kontrolo nismo zaznali letalnih ali subletalnih učinkov. Posledično LC50 ali EC50 ni bilo mogoče določiti. Razlik v delovanju enantiomerov in racemata nismo zaznali pri nobeni izmed testiranih snovi, ravno tako ni bilo razlik v odvisnosti od koncentracije z izjemo statistično značilne razlike v letalnih učinkih pri 0,7 in 6,5 mg/L R-MDMA po 96 h. Med hitrostjo in preplavano potjo larv po 96 urah izpostavljenosti testnim in kontrolnim pogojem nismo zaznali razlik. Naši rezultati ne kažejo na akutno toksičnost AMP, MAMP in MDMA za zarodke cebric pri izbranih koncentracijah in eksperimentalnih pogojih. Human activity is resulting in an increasing presence of environmental pollutants which can have detrimental effects on living organisms, including humans. Illicit drug concentrations of ecotoxicological relevance have been detected in the effluent of wastewater treatment plants. From there they pass into surface, ground- and even drinking water. The aim of this research is to elucidate possible toxic effects of three illicit drugs – amphetamine, methamphetamine and MDMA (ecstasy) – on zebrafish (Danio rerio) embryos. The testing of pure enantiomers and racemates of these drugs was performed following OECD 236 (2013) test guidelines. Additionally, a preliminary ethological essay consisting of movement tracking was performed after 96 hours of exposure. No lethal or sublethal effects were observed after the 96-hour exposure to tested substances at concentration ranging from 0,2 mg/L to 8,6 mg/L compared to negative control. Thus, LC50 or EC50 values could not be determined for any of the substances. No significant differences in the effect of R- and S-enantiomeres was observed in comparison to the racemic mixtures, nor have there been differences in the effects of lower and higher concentrations with the exception of the lethal effects of RMDMA at concentrations 0,7 mg/L and 6,5 mg/L after 96 hours of exposure. No differences in the distance and speed of larvae after 96 hours of exposure were observed in drug-treated larvae compared to negative control. To conclude, the results of our study are not indicative of AMP, MAMP and MDMA having an acute toxic effect on zebrafish embryos. Danio rerio zarodki cebric AMP MAMP MDMA ekotoksikološko testiranje FET amfetamin Danio rerio zebrafish embryo AMP METH MDMA ecotoxicology testing FET amphetamine true false false [E. Cerkvenik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-03-09 07:15:35 2024-03-09 07:15:38 2024-03-11 09:09:11 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 597.2/.5:613.83(043.2) 236335 188302339 38992.pdf 38992.pdf 1 F9E696E0CE2C96F815790883519F0DE7 fa6d4fa30da54edbc129ad09a44f8ee6f600ecf80141e0907bc504eea10c42a6 73082e75-dddc-11ee-85f5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181584 Biotehniška fakulteta 0 0 0