20.500.12556/RUL-154747 Characterization of nanohybridosomes from lipids and spruce homogenate containing extracellular vesicles Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized hybridosomes or nanohybridosomes, NSHs) were considered. Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy, interferometric light microscopy and liquid chromatography–mass spectrometry. Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the particles R$_h$ (between 140 and 180 nm), while the concentrations of the particles were in the range between 10$^{13}$ and 10$^{14}$/mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and proteins were found in hybridosomes. Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that large-scale production of NSHs from lipids and spruce needle homogenate is feasible. hybridosomes liposomes nanovesicles extracellular particles small cellular particles drug delivery hibridosomi liposomi nanovezikli izvencelični vezikli mali celični delci prenos učinkovin true false true Angleški jezik Slovenski jezik Članek v reviji 2024-02-28 10:20:38 2024-02-28 10:20:39 2024-12-20 04:00:42 0000-00-00 00:00:00 2024 0 0 Str. 1709-1721 Vol. 19 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 577:574.5 1178-2013 10.2147/IJN.S432836 186884099 Characterization_of_Nanohybridosomes_from_Lipids_and_Spruce_Homogenate_Containing_Extracellular_Vesicles.pdf Characterization_of_Nanohybridosomes_from_Lipids_and_Spruce_Homogenate_Containing_Extracellular_Vesicles.pdf 1 907756E9D40C0DF9D051A7248300785A 16d1f8a163bb0f721b15358ddcc1ea902ace3e268ff0a9e2e4e3dd06efd2eaea 9a92a879-bdf5-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=197463 https://www.dovepress.com/characterization-of-nanohybridosomes-from-lipids-and-spruce-homogenate-peer-reviewed-fulltext-article-IJN 1 a7f4953e-d61a-11ee-86bb-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181302 https://www.tandfonline.com/doi/full/10.2147/IJN.S432836 1 a824ea2d-d61a-11ee-86bb-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181303 Zdravstvena fakulteta Fakulteta za elektrotehniko Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0