20.500.12556/RUL-154589 Vpliv aktivacije estrogenskih receptorjev pred puberteto na spolno diferenciacijo možganov samic miši The effect of estrogene receptors activation on sexual differentiation of the female mouse brain before puberty Natančni mehanizem vpliva estrogenih hormonov na feminizacijo možganov še ni popolnoma raziskan. Po nekaterih podatkih poteka omenjen proces v obdobju pred puberteto. V naši raziskavi smo samice razdelili v pet skupin. Štirim smo 10. dan po rojstvu odstranili jajčnike in nato od 15. do 25. dneva starosti dodajali snovi, raztopljene v koruznem olju, ki aktivirajo različne signalne poti estrogenih hormonov: 1) estradiol benzoat, 2) agonist estrogenskega receptorja  (ERα) propil pirazol triol, 3) agonist estrogenskega receptorja β diarilpropiontril, 4) koruzno olje (negativna kontrola). Za peto skupino (pozitivno kontrolo) smo uporabili samice, ki so prav tako prejemale koruzno olje, a smo jim odstranili jajčnike šele po puberteti (60. dan starosti). Feminizacijo smo ocenjevali s pomočjo ženskega spolnega obnašanja (lordoze), izražanja tirozin hidroksilaze v anteroventralnem delu periventrikularnega jedra hipotalamusa in ERα v ventromedialnem jedru hipotalamusa. Med skupinami nismo odkrili statistično pomembnih razlik niti v lordozi niti v izražanju omenjenih beljakovin v možganih. Lordoza je bila slabo izražena pri vseh skupinah, posebno pa pri pozitivni kontroli. Naši rezultati kažejo na to, da aktivacija signalnih poti estrogenih hormonov ni trajno vplivala na parametre, ki smo jih raziskovali, torej ni vplivala na feminizacijo. Kljub vsemu pa je velika verjetnost, da so na rezultate vplivali drugi dejavniki, ki bi lahko motili normalni potek aktivne feminizacije, posebno z vidika lordoze. The precise mechanism by which estrogen hormones influence brain feminization is not fully understood. According to some data, this process occurs during the prepubertal period. In our study, we divided female subjects into five groups. Four of them had their ovaries removed on the 10th day after birth, and from the 15th to the 25th day of age, we administered substances dissolved in corn oil that activate various estrogen hormone signaling pathways: 1) estradiol benzoate, 2) agonist of estrogen receptor α (ERα) propyl pyrazole triol, 3) agonist of estrogen receptor β - diarylpropiontril, 4) corn oil (negative control). For the fifth group (positive control), we used females that also received corn oil, but whose ovaries were removed after puberty (at 60 days of age). We evaluated feminization by observing female sexual behavior (lordosis), expression of tyrosine hydroxylase in the anteroventral part of the periventricular nucleus of the hypothalamus, and expression of ERα in the ventromedial nucleus of the hypothalamus. We found no statistically significant differences in lordosis or expression of these proteins in the brains of the different groups. Lordosis was weakly expressed in all groups, especially in the positive control. Our results suggest that activation of estrogen hormone signaling pathways had no lasting effects on the parameters we studied, and thus did not affect feminization. However, it is very likely that other factors influenced the results that may have disrupted the normal course of active feminization, particularly with respect to lordosis. nevroendokrinologija spolna diferenciacija feminizacija možgani spolno vedenje živali lordoza estrogenski receptor euroendocrinology sex differentiation feminization brain sexual behaviour -animal lordosis receptors estrogen true false false A. Brečko Slovenski jezik Angleški jezik Raz. nal. na višji ali visoki šoli 2024-02-22 11:05:53 2024-02-22 11:05:54 2024-02-23 03:44:55 0000-00-00 00:00:00 2023 0 Ljubljana 2023 Ljubljana XI, 65 str. 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612.43:612.82:612.6.057:612.8.05:612.621-31(043.2) 185597955 Amadeja_Brecko_Presernova.pdf Amadeja_Brecko_Presernova.pdf 1 3EE19F0B9192A4B17A5A7CC4087A47A4 5238b595e9b57c8cf80715859e9319b39c44b081db4f5c15a5a02641103abf47 c2846bef-d16a-11ee-bea9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181098 http://knjiznica.vf.uni-lj.si/PortalGenerator/Document.aspx?id=425 1 f72b8b46-d169-11ee-bea9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181097 Veterinarska fakulteta 0 0 0