20.500.12556/RUL-154200 A comparison of the oral microbiota in healthy dogs and dogs with oral tumors The aim of this study was to further describe the oral microbiota of healthy dogs by DNA shotgun sequencing and compare those to dogs with oral tumors. Oral swabs (representative of all niches of the oral cavity) were collected from healthy dogs (n = 24) and from dogs with different oral tumors (n = 7). DNA was extracted from the swabs and shotgun metagenomic sequencing was performed. Only minor differences in microbiota composition were observed between the two groups. At the phylum level, the Bacteroidota, Proteobacteria, Actinobacteriota, Desulfobacterota and Firmicutes were most abundant in both groups. Observed Operational Taxonomic Units—OTUs (species richness) was significantly higher in the healthy patients, but there was no significant difference in the Shannon diversity index between the groups. No significant difference was found in beta diversity between the groups. The core oral microbiota consisted of 67 bacterial species that were identified in all 24 healthy dogs. Our study provides further insight into the composition of the oral microbiota of healthy dogs and in dogs with oral tumors. microbiota DNA neoplasms biopsy dogs true false true Angleški jezik Ni določen Članek v reviji 2024-01-31 14:30:13 2024-01-31 14:30:17 2024-02-02 03:21:39 0000-00-00 00:00:00 2023 0 0 19 str. iss. 23, art. 3594 Vol. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616 2076-2615 10.3390/ani13233594 173949187 A_comparison-Nemec_Ana.pdf A_comparison-Nemec_Ana.pdf 1 E23703242D65F1DBB7C9B6337E112808 5b0ef9409c7308779bcd06e14ab00a614c632384db3383573e5ea3406afb4fe5 f5202cfd-c03d-11ee-a648-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180448 https://www.mdpi.com/2076-2615/13/23/3594 1 df88a7bc-c03c-11ee-a648-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180447 Veterinarska fakulteta 0 0 0