20.500.12556/RUL-154103 CRISPR technologies in Chinese hamster ovary cell line engineering The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and enhanced product quality. This review summarizes recent advances in the application of CRISPR technology for CHO cell line engineering with a particular focus on glycosylation modulation, productivity enhancement, tackling adventitious agents, elimination of problematic host cell proteins, development of antibiotic-free selection systems, site-specific transgene integration, and CRISPR-mediated gene activation and repression. The review highlights the potential of CRISPR technology in CHO cell line genome editing and epigenetic engineering for the more efficient and cost-effective development of biopharmaceuticals while ensuring the safety and quality of the final product. Chinese hamster ovary cells cell line development CRISPR-Cas genome editing glycoengineering augmenting productivity jajčne celice kitajskega hrčka razvoj celične linije urejanje genoma glikoinženiring enocelično zaporedje povečanje produktivnosti genomika true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-25 10:59:03 2024-01-25 10:59:08 2024-01-27 03:18:55 0000-00-00 00:00:00 2023 0 0 26 str. iss. 9, art. 8144 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 575.111 1422-0067 10.3390/ijms24098144 150745347 ijms-24-08144.pdf ijms-24-08144.pdf 1 B3DA1F65C54FBF64E66A585D4AFB1343 c34ccaf6cc2f778c9f21dc6b1ea47878049960a157158ce061b4e485de0b2fa9 fc4e421b-bb68-11ee-9ead-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180279 https://www.mdpi.com/1422-0067/24/9/8144 1 63807db3-bb68-11ee-9ead-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180278 Fakulteta za farmacijo 0 0 0