20.500.12556/RUL-154088 Microfluidic electrochemical glucose biosensor with in situ enzyme immobilization The development and characterization of a microfluidic electrochemical glucose biosensor are presented herein. The transducer part is based on thin-film metal electrodes on a glass substrate. The biological recognition element of the biosensor is the pyrroloquinoline quinone–glucose dehydrogenase (PQQ-GdhB) enzyme, selectively in situ immobilized via microcontact printing of a mixed self-assembling monolayer (SAM) on a gold working electrode, while the microfluidic part of the device comprises microchannel and microfluidic connections formed in a polydimethylsiloxane (PDMS) elastomer. The electrode properties throughout all steps of biosensor construction and the biosensor response to glucose concentration and analyte flow rate were characterized by cyclic voltammetry and chronoamperometry. A measurement range of up to 10 mM in glucose concentration with a linear range up to 200 µM was determined. A detection limit of 30 µM in glucose concentration was obtained. Respective biosensor sensitivities of 0.79 nA/µM/mm$^2$ and 0.61 nA/µM/mm$^2$ were estimated with and without a flow at 20 µL/min. The developed approach of in situ enzyme immobilization can find a wide number of applications in the development of microfluidic biosensors, offering a path towards continuous and time-independent detection. microfluidic glucose biosensor electrochemical biosensor thin film electrode enzyme in situ immobilization chronoamperometry cyclic voltammetry mikrofluidni biosenzor glukoze elektrokemični biosenzor tankoplastna elektroda imobilizacija encima in situ kronoamperometrija ciklična voltametrija true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-24 15:08:54 2024-01-24 15:08:55 2024-01-25 03:42:45 0000-00-00 00:00:00 2023 0 0 16 str. iss. 3, art. 364 Vol. 13 Mar. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.1:53.082.9 2079-6374 10.3390/bios13030364 145284355 biosensors-13-00364-v2.pdf biosensors-13-00364-v2.pdf 1 2254ABD57021F42A64004E12893430F4 75260f22fb039f39aa303794b51de9df2bd24e748ccd34b2cf2d397974111e0e 60b7ff1e-bac2-11ee-bcde-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180254 https://www.mdpi.com/2079-6374/13/3/364 1 1cd66dcc-bac2-11ee-bcde-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180253 Fakulteta za elektrotehniko 0 0 0