20.500.12556/RUL-154040 Dynamics of cell death due to electroporation using different pulse parameters as revealed by diferent viability assays The mechanisms of cell death due to electroporation are still not well understood. Recent studies suggest that cell death due to electroporation is not an immediate all-or-nothing response but rather a dynamic process that occurs over a prolonged period of time. To investigate whether the dynamics of cell death depends on the pulse parameters or cell lines, we exposed different cell lines to different pulses [monopolar millisecond, microsecond, nanosecond, and high-frequency bipolar (HFIRE)] and then assessed viability at different times using different viability assays. The dynamics of cell death was observed by changes in metabolic activity and membrane integrity. In addition, regardless of pulse or cell line, the dynamics of cell death was observed only at high electroporation intensities, i.e., high pulse amplitudes and/or pulse number. Considering the dynamics of cell death, the clonogenic assay should remain the preferred viability assay for assessing viability after electroporation. electroporation pulsed field ablation cell death dynamics pulse parameters viability assays elektroporacija ablacija s pulznim električnim poljem dinamika celične smrti parametri pulza testi preživetja true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-22 08:55:46 2024-01-22 08:55:47 2024-01-23 03:38:30 0000-00-00 00:00:00 2024 0 0 Str. 22-35 iss. 1 Vol. 52 Jan. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621 0090-6964 10.1007/s10439-023-03309-8 164795907 s10439-023-03309-8.pdf s10439-023-03309-8.pdf 1 5F91A7C1C169A2867117AEC25DFD0A78 5159cfd12832741eb0456b08e2ae4af9281dd1a36f8fe3559b3f97841e013a25 0cd50197-b8fc-11ee-88cd-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180178 https://link.springer.com/article/10.1007/s10439-023-03309-8 1 a82a4ec9-b8fb-11ee-88cd-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180175 Fakulteta za elektrotehniko 0 0 0