20.500.12556/RUL-154019 A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis Bacillus thuringiensis serovar israelensis is the most widely used biopesticide against insects, including vectors of animal and human diseases. Among several extrachromosomal elements, this endospore-forming entomopathogen harbors two bacteriophages: a linear DNA replicon named GIL01 that does not integrate into the chromosome during lysogeny and a circular-jumbo prophage known as pBtic235. Here, we show that GIL01 hinders the induction of cohabiting prophage pBtic235. The GIL01-encoded small protein, gp7, which interacts with the host LexA repressor, is a global transcription regulator and represses the induction of pBtic235 after DNA damage to presumably allow GIL01 to multiply first. In a complex with host LexA in stressed cells, gp7 down-regulates the expression of more than 250 host and pBtic235 genes, many of which are involved in the cellular functions of genome maintenance, cell-wall transport, and membrane and protein stability. We show that gp7 homologs that are found exclusively in bacteriophages act in a similar fashion to enhance LexA’s binding to DNA, while likely also affecting host gene expression. Our results provide evidence that GIL01 influences both its host and its co-resident bacteriophage. bacteriophages DNA damage DNA repair bakteriofagi Bacillus thuringiensis odziv SOS profagi true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-19 12:36:44 2024-01-19 12:36:44 2024-01-20 03:43:25 0000-00-00 00:00:00 2022 0 0 12 str. Vol. 5, art. 1286 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.6 2399-3642 10.1038/s42003-022-04238-3 132540419 s42003-022-04238-3.pdf s42003-022-04238-3.pdf 1 19D5B8344D9D2E42496C10B1A940F647 087e86d12e1c390ff4c669e909363363e7133bb6dcfb8f8c13bf97cc35fb8218 f4f747ba-b6bf-11ee-b009-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180150 https://www.nature.com/articles/s42003-022-04238-3 0 37283187-b6bf-11ee-b009-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180149 Biotehniška fakulteta 0 0 0