20.500.12556/RUL-153973 Nutrient availability and biofilm polysaccharide shape the bacillaene-dependent antagonism of Bacillus subtilis against Salmonella Typhimurium Salmonella enterica is one of the most common foodborne pathogens and, due to the spread of antibiotic resistance, new antimicrobial strategies are urgently needed to control it. In this study, we explored the probiotic potential of Bacillus subtilis PS-216 and elucidated the mechanisms that underlie the interactions between this soil isolate and the model pathogenic strain S. Typhimurium SL1344. The results reveal that B. subtilis PS-216 inhibits the growth and biofilm formation of S. Typhimurium through the production of the pks cluster-dependent polyketide bacillaene. The presence of S. Typhimurium enhanced the activity of the P$_{pksC}$ promoter that controls bacillaene production, suggesting that B. subtilis senses and responds to Salmonella. The level of Salmonella inhibition, overall P$_{pksC}$ activity, and P$_{pksC}$ induction by Salmonella were all higher in nutrient-rich conditions than in nutrient-depleted conditions. Although eliminating the extracellular polysaccharide production of B. subtilis via deletion of the epsA-O operon had no significant effect on inhibitory activity against Salmonella in nutrient-rich conditions, this deletion mutant showed an enhanced antagonism against Salmonella in nutrient-depleted conditions, revealing an intricate relationship between exopolysaccharide production, nutrient availability, and bacillaene synthesis. Overall, this work provides evidence on the regulatory role of nutrient availability, sensing of the competitor, and EpsA-O polysaccharide in the social outcome of bacillaene-dependent competition between B. subtilis and S. Typhimurium. Bacillus subtilis Salmonella enterica probiotics enteric pathogen biofilm polysaccharides microbial competition nutrients probiotiki socialne interakcije patogeni mikroorganizmi biofilm true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-17 10:37:00 2024-01-17 10:37:07 2024-01-18 03:42:11 0000-00-00 00:00:00 2022 0 0 14 str. iss. 6 Vol. 10 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26 2165-0497 10.1128/spectrum.01836-22 128780803 podnar-et-al-2022-nutrient-availability-and-biofilm-polysaccharide-shape-the-bacillaene-dependent-antagonism-of.pdf podnar-et-al-2022-nutrient-availability-and-biofilm-polysaccharide-shape-the-bacillaene-dependent-antagonism-of.pdf 1 103CD47172407223C8C9538ACDE47F03 246b2935ec9c3f1782c0232ab5a93da0cbadd8bbbb52a18fdadbdd3c76b86989 792bfd3b-b51c-11ee-b3d9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180064 https://journals.asm.org/doi/10.1128/spectrum.01836-22 1 fb2e6455-b51b-11ee-b3d9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180063 Biotehniška fakulteta 0 0 0