20.500.12556/RUL-153970 Enzymatic treatments of raw, germinated and fermented spelt (Triticum spelta L.) seeds improve the accessibility and antioxidant activity of their phenolics Our study aimed to investigate the effect of cellulase, xylanase, esterase, α-amylase and protease on the enhanced release of phenolics from spelt seeds, and to demonstrate the co-effect of enzymatic treatment, germination or fermentation on the spelt phenolics content and their in vitro and cellular antioxidant activities. Bound phenolics of spelt seeds were successfully released by different combinations of cellulase, xylanase, feruloyl esterase, α-amylase and protease. Samples treated with all five enzymes together showed significantly improved extractable p-coumaric, ferulic and p-hydroxybenzoic acid contents, particularly efficient antioxidant activity in ABTS$^{•+}$ scavenging activity and the highest extractable TPCs. By combining enzymatic treatment with germination and fermentation, extractable TPCs were increased by 28% and 458%, respectively. A combination of enzymatic treatment with germination and fermentation led to enormously increased extractable trans-ferulic acid content (5899% and 8263%, respectively). The bound fraction of enzymatic treated raw seeds showed lower decrease in intracellular oxidation than the corresponding extractable fraction, but a 24-fold higher uptake of hydroxycinnamic acids. Principal component analysis showed some similarities between extractable and bound fractions of enzymatic treated raw, germinated and fermented samples, mainly due to the increase in extractable acids content (biochemical parameters), especially cis-ferulic, caffeic and p-hydroxybenzoic acids. spelt seeds hydrolytic enzymes extractable and bound phenolics cellular antioxidant activity HPLC-MS pira Triticum spelta L. fenolne spojine antioksidativna učinkovitost hidrolitični encimi true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-17 09:17:29 2024-01-17 09:17:33 2024-01-18 03:42:11 0000-00-00 00:00:00 2022 0 0 13 str. Vol. 169, art. 114046 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.111.5:547.56 0023-6438 10.1016/j.lwt.2022.114046 124499715 1-s2.0-S0023643822009811-main.pdf 1-s2.0-S0023643822009811-main.pdf 1 A9E8EF4B735DB5EEED5E208F04A08059 cb0be45710c75dda5b4b0657309b0a44ca6fe0df47e20d79a70fac04b6a9273e 92d1e14b-b511-11ee-b3d9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180057 https://www.sciencedirect.com/science/article/pii/S0023643822009811 0 24b77f21-b511-11ee-b3d9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180056 Biotehniška fakulteta 0 0 0