20.500.12556/RUL-153559 Isolation and characterization of highly active keratinolytic microorganisms with promising potential for waste sheep wool processing The huge amounts of keratin-rich waste generated daily by various industries, slaughterhouses, and processing plants need to be properly managed. Most keratin degradation-related research focuses on keratin from bird feathers, but a vast minority focuses on keratin from sheep wool, which also presents a serious environmental problem. In this article, we describe the isolation, identification, and characterization of new keratinolytic microorganisms capable of sheep wool degradation from sheep wool and soil enriched with wool keratin. We isolated four bacterial species from the genus Bacillus (B. subtilis, B. altitudinis, B. mycoides, and B. wiedmannii), one streptomycete species Streptomyces coelicofavus identified by whole genome sequencing, and a fungal species Aphanoascus reticulisporus. In some of the isolated microorganisms, we detected keratinolytic activity for the first time, and for most of them, the ability to degrade sheep wool has not been previously demonstrated. The keratinases of the new isolates are active in a wide range of temperatures (25–85 °C) and pH (6.0–10.0), so all isolates show great potential for further biotechnological use in industry and in various environmental and agricultural applications to reduce and recycle keratin-rich wastes such as sheep wool and waste woollen textiles. bacteria fungi keratin keratinase sheep wool mikrobiologija keratinolitični mikroorganizmi odpadna ovčja volna true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-15 14:48:54 2024-01-15 14:48:55 2024-09-30 13:18:50 0000-00-00 00:00:00 2024 0 0 Str. 360–372 iss. 1 Vol. 26 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 1611-8227 10.1007/s10163-023-01830-5 171178243 RAZ_Petek_Blaz_2023.pdf RAZ_Petek_Blaz_2023.pdf 1 F27E1E8393C2D3CB69BC80438BCC5372 6d7b559d94685d9848ac1e92d8621a030ff5b3c60c61f00f47ce14e398f05aab d4a2c980-b3ac-11ee-88ef-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179619 https://link.springer.com/article/10.1007/s10163-023-01830-5 0 a7d68ee2-b3ad-11ee-88ef-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179620 Biotehniška fakulteta 0 0 0